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A logic gate-based ...
A logic gate-based fluorogenic probe for Hg2+ detection and its applications in cellular imaging
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- Hu, Jiwen (författare)
- Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten,Tongji University, Peoples R China
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- Hu, Zhang-Jun (författare)
- Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten,Shanghai University, Peoples R China
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- Chen, Zhiwen (författare)
- Shanghai University, Peoples R China
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- Gao, Hong-Wen (författare)
- Tongji University, Peoples R China
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- Uvdal, Kajsa (författare)
- Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten
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(creator_code:org_t)
- ELSEVIER SCIENCE BV, 2016
- 2016
- Engelska.
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Ingår i: Analytica Chimica Acta. - : ELSEVIER SCIENCE BV. - 0003-2670 .- 1873-4324. ; 919, s. 85-93
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A new colorimetric and fluorogenic probe (RN3) based on rhodamine-B has been successfully designed and synthesized. It displays a selective response to Hg2+ in the aqueous buffer solution over the other competing metals. Upon addition of Hg2+, the solution of RN3 exhibits a naked eye observable color change from colorless to red and an intensive fluorescence with about 105-fold enhancement. The changes in the color and fluorescence are ascribed to the ring-opening of spirolactam in rhodamine fluorophore, which is induced by a binding of the constructed receptor to Hg2+ with the association and dissociation constants of 0.22 x 10(5) M-1 and 25.2 mM, respectively. The Jobs plot experiment determines a 1: 1 binding stoichiometry between RN3 and Hg2+. The resultant "turn-on" fluorescence in buffer solution, allows the application of a method to determine Hg2+ levels in the range of 4.0-15.0 mu M, with the limit of detection (LOD) calculated at 60.7 nM (3 sigma/slope). In addition, the fluorescence turn-off and color fading-out happen to the mixture of RN3-Hg2+ by further addition of I- or S2-. The reversible switching cycles of fluorescence intensity upon alternate additions of Hg2+ and S2- demonstrate that RN3 can perform as an INHIBIT logic gate. Furthermore, the potential of RN3 as a fluorescent probe has been demonstrated for cellular imaging. (C) 2016 Elsevier B.V. All rights reserved.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Mercury; Rhodamine; Fluorogenic probe; Fluorescent imaging; Logical gate
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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