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Sökning: id:"swepub:oai:DiVA.org:liu-141185" > Immunization with H...

Immunization with HIV-1 envelope T20-encoding DNA vaccines elicits cross-clade neutralizing antibody responses.

Stenler, S (författare)
Karolinska Institutet,Karolinska Cell Therapy Center, Karolinska University Hospital , 141 86 Stockholm , Sweden,Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet , 171 77 Stockholm , Sweden
Lundin, K E (författare)
Karolinska Institutet,Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet , SE-141 86 Huddinge , Sweden
Hansen, L (författare)
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet , 171 77 Stockholm , Sweden
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Petkov, S (författare)
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet , 171 77 Stockholm , Sweden
Mozafari, N (författare)
Karolinska Institutet,Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet , SE-141 86 Huddinge , Sweden
Isaguliants, M (författare)
Karolinska Institutet,Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet , 171 77 Stockholm , Sweden
Blomberg, P (författare)
Karolinska Institutet,Karolinska Cell Therapy Center, Karolinska University Hospital , 141 86 Stockholm , Sweden
Smith, C I E (författare)
Karolinska Institutet,Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet , SE-141 86 Huddinge , Sweden
Goldenberg, D M (författare)
Immunomedics, Inc.; IBC Pharmaceuticals, Inc., Morris Plains, New Jersey 07950 , USA
Chang, C-H (författare)
Immunomedics, Inc.; IBC Pharmaceuticals, Inc., Morris Plains, New Jersey 07950 , USA
Ljungberg, K (författare)
Karolinska Institutet,Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet , 171 77 Stockholm , Sweden
Hinkula, Jorma (författare)
Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten
Wahren, B (författare)
Karolinska Institutet,Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet , 171 77 Stockholm , Sweden
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 (creator_code:org_t)
2017-07-11
2017
Engelska.
Ingår i: Human Vaccines & Immunotherapeutics. - : Taylor & Francis. - 2164-5515 .- 2164-554X. ; 13:12, s. 2849-2858
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • BACKGROUND: Genetic immunization is expected to induce the expression of antigens in a native form. The encoded peptide epitopes are presented on endogenous MHC molecules, mimicking antigen presentation during a viral infection. We have explored the potential of enfuvirtide (T20), a short HIV peptide with antiviral properties, to enhance immune response to HIV antigens. To generate an expression vector, the T20 sequence was cloned into a conventional plasmid, the novel minicircle construct, and a replicon plasmid. In addition, three conventional plasmids that express the envelope of HIV-1 subtypes A, B and C and contain T20 in their gp41 sequences were also tested.RESULTS: All combinations induced HIV-specific antibodies and cellular responses. The addition of T20 as a peptide and as an expression cassette in the three DNA vectors enhanced antibody responses. The highest anti-HIV-1 Env titers were obtained by the replicon T20 construct. This demonstrates that besides its known antiviral activity, T20 promotes immune responses. We also confirm that the combination of slightly divergent antigens improves immune responses.CONCLUSIONS: The antiretroviral T20 HIV-1 sequence can be used as an immunogen to elicit binding and neutralizing antibodies against HIV-1. These, or similarly modified gp41 genes/peptides, can be used as priming or boosting components for induction of broadly neutralizing anti-HIV antibodies. Future comparative studies will reveal the optimal mode of T20 administration.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

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