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A systematic multic...
A systematic multicellular spheroids screening approach lead to the identification of antineoplastic activity in three different plant extracts from the Egyptian flora
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- Fayad, W. (författare)
- Drug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, National Research Centre, Pharmaceutical and Drug Industries Division
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- El-Hallouty, S.M. (författare)
- Drug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, National Research Centre, Pharmaceutical and Drug Industries Division
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- El-Manawaty, M.A. (författare)
- Drug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, National Research Centre, Pharmaceutical and Drug Industries Division
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- Mounier, M.M. (författare)
- Drug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, National Research Centre, Pharmaceutical and Drug Industries Division
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- Soliman, A.A.F. (författare)
- Drug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, National Research Centre, Pharmaceutical and Drug Industries Division
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- Mahmoud, K. (författare)
- Drug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, National Research Centre, Pharmaceutical and Drug Industries Division
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- Yousry, A.A. (författare)
- Medicinal and Aromatic Plants Department, Pharmaceutical and Drug Industries Division, National Research Centre
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- Farghaly, A.A. (författare)
- Genetics and Cytology Department, National Research Centre
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- Fahmy, M.A. (författare)
- Genetics and Cytology Department, National Research Centre
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- Hasasn, Z.M. (författare)
- Chemistry of Natural Compounds Department, National Research Centre
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- Linder, Stig (författare)
- Linköpings universitet,Avdelningen för läkemedelsforskning,Medicinska fakulteten,Cancer Center Karolinska, Department of Oncology and Pathology, Karolinska Institute
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(creator_code:org_t)
- Medipoeia Publication, 2017
- 2017
- Engelska.
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Ingår i: Journal of Applied Pharmaceutical Science. - : Medipoeia Publication. - 2231-3354. ; 7:6, s. 13-22
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.7...
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Abstract
Ämnesord
Stäng
- Developing natural products as potential antineoplastic drugs is a meticulous process involving both compound isolation and biological testing. Many studies are based on primary screening using tumor cell viability as the readout followed by compound isolation. We here present an approach which utilizes both 2-D and 3-D cultured of tumor cells for screening and immortalized human non-transformed cells for counter screening. This procedure increases the precision of identifying tumor-specific cytotoxic compounds with interesting pharmacological properties. Using this straight-forward approach, we screened 500 plant extracts from the Egyptian flora for anticancer activity. The primary screen on 2-D cultured cells yielded 41 hits, 12 of which showed significant cytotoxicity on 3-D cultured cells. Of these, 4 extracts showed limited cytoxicity to normal cells. We conclude that only ~10% of the cytotoxic extracts showed desired properties with regard to tumor parenchyme penetration and tumor-specific activity. Extracts from Euphorbia dendroides L. herb, Ononis vaginalis Vahl. herb and Quercus robur L. branches were found to induce tumor apoptosis and were considered the most promising. These three extracts showed significant inhibition in the Ehrlich ascites carcinoma in vivo model and did not show severe toxicity on healthy animals.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Nyckelord
- Anticancer
- Multicellular spheroids
- Plant extracts
- Pro-apoptotic
- Screening
- Therapeutic window
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Fayad, W.
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El-Hallouty, S.M ...
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El-Manawaty, M.A ...
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Mounier, M.M.
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Soliman, A.A.F.
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Mahmoud, K.
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visa fler...
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Yousry, A.A.
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Farghaly, A.A.
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Fahmy, M.A.
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Hasasn, Z.M.
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Linder, Stig
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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Linköpings universitet