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Persistent Wnt/β‐Ca...
Persistent Wnt/β‐Catenin Signaling Determines Dorsalization of the Postnatal Subventricular Zone and Neural Stem Cell Specification into Oligodendrocytes and Glutamatergic Neurons
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- Azim, Kasum (författare)
- Brain Research Institute, University of Zürich/ETHZ, Zürich, Switzerland
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- Fischer, Bruno (författare)
- Brain Research Institute, University of Zürich/ETHZ, Zürich, Switzerland
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- Hurtado-Chong, Anahi (författare)
- Brain Research Institute, University of Zürich/ETHZ, Zürich, Switzerland
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- Draganova, Kalina (författare)
- Institute of Anatomy, University of Zürich, Switzerland
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- Cantù, Claudio (författare)
- Institute of Molecular Life Sciences, University of Zürich, Switzerland
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- Zemke, Martina (författare)
- Institute of Anatomy, University of Zürich, Switzerland
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- Sommer, Lukas (författare)
- Institute of Anatomy, University of Zürich, Switzerland
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- Butt, Arthur (författare)
- Institute of Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, Portsmouth University, United Kingdom
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- Raineteau, Olivier (författare)
- Brain Research Institute, University of Zürich/ETHZ, Zürich, Switzerland / Stem Cell and Brain Research Institute, INSERMU846, Lyon, France, Universite de Lyon,Universite Lyon, France
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(creator_code:org_t)
- 2014-04-17
- 2014
- Engelska.
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Ingår i: Stem Cells. - Durham, United States : AlphaMed Press, Inc.. - 1066-5099 .- 1549-4918. ; 32:5, s. 1301-1312
- Relaterad länk:
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https://doi.org/10.1...
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https://stemcellsjou...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In the postnatal and adult central nervous system (CNS), the subventricular zone (SVZ) of the forebrain is the main source of neural stem cells (NSCs) that generate olfactory neurons and oligodendrocytes (OLs), the myelinating cells of the CNS. Here, we provide evidence of a primary role for canonical Wnt/β-catenin signaling in regulating NSC fate along neuronal and oligodendroglial lineages in the postnatal SVZ. Our findings demonstrate that glutamatergic neuronal precursors (NPs) and oligodendrocyte precursors (OPs) are derived strictly from the dorsal SVZ (dSVZ) microdomain under the control of Wnt/β-catenin, whereas GABAergic NPs are derived mainly from the lateral SVZ (lSVZ) microdomain independent of Wnt/β-catenin. Transcript analysis of microdissected SVZ microdomains revealed that canonical Wnt/β-catenin signaling was more pronounced in the dSVZ microdomain. This was confirmed using the β-catenin-activated Wnt-reporter mouse and by pharmacological stimulation of Wnt/β-catenin by infusion of the specific glycogen synthase kinase 3β inhibitor, AR-A014418, which profoundly increased the generation of cycling cells. In vivo genetic/pharmacological stimulation or inhibition of Wnt/β-catenin, respectively, increased and decreased the differentiation of dSVZ-NSCs into glutamatergic NPs, and had a converse effect on GABAergic NPs. Activation of Wnt/β-catenin dramatically stimulated the generation of OPs, but its inhibition had no effect, indicating other factors act in concert with Wnt/β-catenin to fine tune oligodendrogliogenesis in the postnatal dSVZ. These results demonstrate a role for Wnt/β-catenin signaling within the dorsal microdomain of the postnatal SVZ, in regulating the genesis of glutamatergic neurons and OLs.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Cell signaling
- Nervous system
- Neural induction
- Neural stem cell
- Neuron
- Oligodendrocytes
- Progenitor cells
- Stem cell plasticity
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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