Evaluation of potential anti-cancer activity of cationic liposomal nanoformulated Lycopodium clavatum in colon cancer cells

• Research dealing with early diagnosis and efficient treatment in colon cancer to improve patients survival is still under investigation. Chemotherapeutic agent result in high systemic toxicity due to their non-specific actions on DNA repair and/or cell replication. Traditional medicine such as Lycopodium clavatum (LC) has been claimed to have therapeutic potentials against cancer. The present study focuses on targeted drug delivery of cationic liposomal nanoformulated LC (CL-LC) in colon cancer cells (HCT15) and comparing the efficacy with an anti-colon cancer drug, 7-ethyl-10-hydroxy-camptothecin (SN38) along with its nanoformulated form (CL-SN38). The colloidal suspension of LC was made using thin film hydration method. The drugs were characterised using ultraviolet, dynamic light scattering, scanning electron microscopy, energy, dispersive X-ray spectroscopy. Invitro drug release showed kinetics of 49 and 89% of SN38 and LC, whereas CL-SN38 and CL-LC showed 73 and 74% of sustained drug release, respectively. Studies on morphological changes, cell viability, cytotoxicity, apoptosis, cancer-associated gene expression analysis of Bcl-2, Bax, p53 by real-time polymerase chain reaction and western blot analysis of Bad and p53 protein were performed. Nanoformulated LC significantly inhibited growth and increased the apoptosis of colon cancer cells indicating its potential anti-cancer activity against colon cancer cells.

Ämnesord

NATURVETENSKAP  -- Fysik -- Den kondenserade materiens fysik (hsv//swe)

NATURAL SCIENCES  -- Physical Sciences -- Condensed Matter Physics (hsv//eng)

Nyckelord

cancer; biological organs; cellular biophysics; drug delivery systems; drugs; nanomedicine; genetics; DNA; molecular biophysics; biochemistry; lipid bilayers; toxicology; suspensions; colloids; light scattering; X-ray chemical analysis; solvation; enzymes; nanostructured materials; energy dispersive X-ray spectroscopy; in vitro drug release; morphological changes; cell viability; cytotoxicity; apoptosis; cancer-associated gene expression analysis; Bcl-2; Bax; real-time polymerase chain reaction; western blot analysis; Bad protein; p53 protein; scanning electron microscopy; dynamic light scattering; ultraviolet scattering; thin film hydration method; colloidal suspension; nanoformulated form CL-SN38; 7-ethyl-10-hydroxy-camptothecin; anticolon cancer drug; colon cancer cells HCT15; cationic liposomal nanoformulated LC; targeted drug delivery; therapeutic potentials; Lycopodium clavatum; traditional medicines; cell replication; DNA repair; nonspecific actions; high systemic toxicity; chemotherapeutic agents; patient survival; colon cancer treatment; colon cancer diagnosis; CL-LC; potential anticancer activity

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