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Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads
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- Lee, Seung Joon (författare)
- Department of New Biology, DGIST, Daegu, Republic of Korea; CytoDx, Pangyo-ro, Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
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- Sim, Tae Seok (författare)
- Samsung Electronics, Ltd., Maetan3-dong, Youngtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea
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- Shin, Hyun Young (författare)
- Department of New Biology, DGIST, Daegu, Republic of Korea
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- Department of New Biology, DGIST, Daegu, Republic of Korea; CytoDx, Pangyo-ro, Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea Samsung Electronics, Ltd, Maetan3-dong, Youngtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea (creator_code:org_t)
- 2019-10-24
- 2019
- Engelska.
- Ingår i: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 14:10
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Microchips are widely used to separate circulating tumor cells (CTCs) from whole blood by virtues of sophisticated manipulation for microparticles. Here, we present a chip with an 8 µm high and 27.9 mm wide slit to capture cancer cells bound to 3 µm beads. Apart from a higher purity and recovery rate, the slit design allows for simplified fabrication, easy cell imaging, less clogging, lower chamber pressure and, therefore, higher throughput. The beads were conjugated with anti-epithelial cell adhesion molecules (anti-EpCAM) to selectively bind to breast cancer cells (MCF-7) used to spike the whole blood. The diameter of the cell-bead construct was in average 23.1 µm, making them separable from other cells in the blood. As a result, the cancer cells were separated from 5 mL of whole blood with a purity of 52.0% and a recovery rate of 91.1%, and also we confirmed that the device can be applicable to clinical samples of human breast cancer patients. The simple design with microslit, by eliminating any high-aspect ratio features, is expected to reduce possible defects on the chip and, therefore, more suitable for mass production without false separation outputs.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
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- Lee, Seung Joon
- Sim, Tae Seok
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- Sunoo, Joseph
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- Lee, Jeong-Gun
- Yea, Kyungmoo
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- Kim, Woon-Hae
- Park, Kyun Woo
- Kim, Minseok S
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