Search: id:"swepub:oai:DiVA.org:liu-190218" >
A Novel High Discri...
A Novel High Discriminatory Protocol for the Detection of Borrelia afzelii, Borrelia burgdorferi Sensu Stricto and Borrelia garinii in Ticks
-
- Chiappa, Giulia (author)
- Univ Milan, Italy
-
- Perini, Matteo (author)
- Univ Milan, Italy
-
- Cafiso, Alessandra (author)
- Univ Milan, Italy
-
show more...
-
- Nodari, Riccardo (author)
- Univ Milan, Italy
-
- Wilhelmsson, Peter (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Dept Clin Microbiol, Sweden
-
- Lindgren, Per-Eric (author)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Dept Clin Microbiol, Sweden
-
- Omazic, Anna (author)
- Natl Vet Inst, Sweden
-
- Ullman, Karin (author)
- Natl Vet Inst, Sweden
-
- Moutailler, Sara (author)
- Ecole Natl Vet Alfort, France
-
- Kjellander, Petter (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för ekologi,Department of Ecology,Swedish Univ Agr Sci, Sweden
-
- Bazzocchi, Chiara (author)
- Univ Milan, Italy
-
- Grandi, Giulio (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,National Veterinary Institute SVA,Natl Vet Inst, Sweden; Swedish Univ Agr Sci, Sweden
-
show less...
-
(creator_code:org_t)
-
- 2022-10-25
- 2022
- English.
-
In: Pathogens. - : MDPI. - 2076-0817. ; 11:11
- Related links:
-
https://liu.diva-por... (primary) (Raw object)
-
show more...
-
https://pub.epsilon.... (primary) (Raw object) (free)
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
https://res.slu.se/i...
-
show less...
Abstract
Subject headings
Close
- Bacteria of the Borrelia burgdorferi sensu lato complex are the causative agents of Lyme borreliosis (LB). Even if the conventional diagnosis of LB does not rely on the species itself, an accurate species identification within the complex will provide a deepened epidemiological scenario, a better diagnosis leading to a more targeted therapeutic approach, as well as promote the general publics awareness. A comparative genomics approach based on the 210 Borrelia spp. genomes available in 2019 were used to set up three species-specific PCR protocols, able to detect and provide species typing of Borrelia afzelii, Borrelia burgdorferi sensu stricto (s.s.) and Borrelia garinii, the three most common and important human pathogenic Lyme Borrelia species in Europe. The species-specificity of these protocols was confirmed on previously identified B. afzelii, B. burgdorferi s.s. and B. garinii specimens detected in Ixodes ricinus samples. In addition, the protocols were validated on 120 DNA samples from ticks collected in Sweden, showing 88% accuracy, 100% precision, 72% sensitivity and 100% specificity. The proposed approach represents an innovative tool in epidemiological studies focused on B. burgdorferi s.l. occurrence in ticks, and future studies could suggest its helpfulness in routine diagnostic tests for health care.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biologisk systematik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biological Systematics (hsv//eng)
Keyword
- Borrelia burgdorferi sensu lato; molecular typing; real time-PCR; Ixodes
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
Pathogens
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Chiappa, Giulia
-
Perini, Matteo
-
Cafiso, Alessand ...
-
Nodari, Riccardo
-
Wilhelmsson, Pet ...
-
Lindgren, Per-Er ...
-
show more...
-
Omazic, Anna
-
Ullman, Karin
-
Moutailler, Sara
-
Kjellander, Pett ...
-
Bazzocchi, Chiar ...
-
Grandi, Giulio
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Infectious Medic ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Microbiology
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biological Syste ...
- Articles in the publication
-
Pathogens
- By the university
-
Linköping University
-
Swedish University of Agricultural Sciences