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Fabrication and Cha...
Fabrication and Characterization of Dextranase Nano-Entrapped Enzymes in Polymeric Particles Using a Novel Ultrasonication-Microwave Approach
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- Bashari, Mohanad (författare)
- Asharqiah Univ, Oman
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- Ahmed, Hani (författare)
- Nanchang Univ, Peoples R China
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- Mustafa, Ayman Balla (författare)
- Misurata Univ, Libya
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- Riaz, Asad (författare)
- United Arab Emirates Univ, U Arab Emirates
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- Wang, Jinpeng (författare)
- Beijing Technol & Business Univ BTBU, Peoples R China
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- Saddick, Salina Yahya (författare)
- King Abdulaziz Univ, Saudi Arabia
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- Omar, Abdulkader Shaikh (författare)
- King Abdulaziz Univ, Saudi Arabia; Najla Bint Saud Al Saud Ctr Distinguished Res Biot, Saudi Arabia
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- Afifi, Mohamed (författare)
- Najla Bint Saud Al Saud Ctr Distinguished Res Biot, Saudi Arabia; Univ Jeddah, Saudi Arabia
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- Al-Farga, Ammar (författare)
- Univ Jeddah, Saudi Arabia
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- AlJumaiah, Lulwah Zeyad (författare)
- Univ Hail, Saudi Arabia
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- Abourehab, Mohammed A. S. (författare)
- Umm Al Qura Univ, Saudi Arabia
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- Belal, Amany (författare)
- Beni Suef Univ, Egypt; Taif Univ, Saudi Arabia
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- Khalifa, Mohamed Yassin Zaky (författare)
- Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Region Östergötland, Onkologiska kliniken US,Beni Suef Univ, Egypt
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(creator_code:org_t)
- 2023-01-05
- 2023
- Engelska.
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Ingår i: Catalysts. - : MDPI. - 2073-4344. ; 13:1
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- In the current study, a novel method to improve the nano-entrapment of enzymes into Ca-alginate gel was investigated to determine the synergistic effects of ultrasound combined with microwave shock (UMS). The effects of UMS treatment on dextranase enzymes loading effectiveness (LE) and immobilization yield (IY) were investigated. By using FT-IR spectra and SEM, the microstructure of the immobilized enzyme (IE) was characterized. Additionally, the free enzyme was used as a control to compare the reusability and enzyme-kinetics characteristics of IEs produced with and without UMS treatments. The results demonstrated that the highest LE and IY were obtained when the IE was produced with a US of 40 W at 25 kHz for 15 min combined with an MS of 60 W at a shock rate of 20 s/min for 20 min, increasing the LE and the IY by 97.32 and 78.25%, respectively, when compared with an immobilized enzyme prepared without UMS treatment. In comparison with the control, UMS treatment dramatically raised the Vmax, KM, catalytic, and specificity constant values for the IE. The outcomes suggested that a microwave shock and ultrasound combination would be an efficient way to improve the immobilization of enzymes in biopolymer gel.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Nyckelord
- nano-entrapment; ultrasound; microwave shock; immobilization; dextranase; Ca-alginate nanoparticles; purification; molecular mass; degradation; clinical applications
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Bashari, Mohanad
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Ahmed, Hani
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Mustafa, Ayman B ...
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Riaz, Asad
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Wang, Jinpeng
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Saddick, Salina ...
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Omar, Abdulkader ...
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Afifi, Mohamed
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Al-Farga, Ammar
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AlJumaiah, Lulwa ...
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Abourehab, Moham ...
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Belal, Amany
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Khalifa, Mohamed ...
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