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Immunolocalization ...
Immunolocalization of Cholecystokinin-2 Receptors in Rat Gastric Mucosa
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- Tømmerås, Karin (författare)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
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- Hammer, Pål (författare)
- Linköpings universitet,Institutionen för biomedicin och kirurgi,Hälsouniversitetet
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- Sundler, Frank (författare)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine,Department of Physiological Sciences, Section of Neuroendocrine Cell Biology, Lund University, Lund, Sweden
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- Borch, Kurt, 1944- (författare)
- Linköpings universitet,Institutionen för biomedicin och kirurgi,Hälsouniversitetet
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- Mårdh, Sven, 1947- (författare)
- Linköpings universitet,Institutionen för biomedicin och kirurgi,Hälsouniversitetet
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- Cabero, José Lius (författare)
- Discovery, Research Area CV & GI, AstraZeneca R&D Mölndal, Mölndal, Sweden
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(creator_code:org_t)
- 2009-07-08
- 2002
- Engelska.
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Ingår i: Scandinavian Journal of Gastroenterology. - : Informa UK Limited. - 0036-5521 .- 1502-7708. ; 37:9, s. 1017-1024
- Relaterad länk:
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- BACKGROUND: Gastrin exerts trophic effects on the gastric mucosa by mechanisms not yet completely elucidated. Our aim was to localize the cholecystokinin-2 (CCK2) receptor in epithelial cells of foetal and adult rat stomachs in order to determine the cell types that are directly affected by gastrin.METHODS: Gastric tissue was subjected to indirect double immunofluorescence staining with antiserum against the C-terminal decapeptide of the CCK2 receptor and antibodies against 5' bromo-2-deoxyuridine, which had been injected into the rats I h before they were killed, the acid pump H,K-ATPase, the membrane-cytoskeletal linker ezrin, pepsin/pepsinogen or histidine decarboxylase.RESULTS: Undifferentiated foetal gastric epithelial cells expressed CCK2 receptors, whereas stem cells of adult gastric glands did not exhibit immunoreactivity. However, other epithelial cells in the progenitor zone of adult gastric glands did express CCK2 receptors. Some of these cells were faintly stained for H,K-ATPase; pepsin/pepsinogen was also detected in this region. Parietal cells in the isthmus/pit region of the glands contained ezrin, and some showed weak immunoreactivity for the CCK2 receptor. As expected, enterochromaffin-like cells also expressed CCK2 receptors.CONCLUSION: Our findings are consistent with the hypothesis that a CCK2 receptor mediates direct effects of gastrin on gastric epithelial cells during both stomach organogenesis and adult life.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
Nyckelord
- Cck2
- Receptor
- Gastrin
- Immunofluorescence
- Organogenesis
- Progenitor Zone
- Stomach
- MEDICINE
- MEDICIN
- organogenesis
- immunofluorescence
- CCK2 receptor
- gastrin
- progenitor
- zone
- stomach
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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