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Effects of lactofer...
Effects of lactoferrin on rat dermal interstitial fluid pressure (Pif) and in vitro endothelial barrier function
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Erga, KS (författare)
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Peen, E (författare)
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- Eneström, S (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Molekylär och immunologisk patologi,Klinisk patologi och klinisk genetik
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visa fler...
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Reed, RK (författare)
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visa färre...
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: Acta Physiologica Scandinavica. - 0001-6772 .- 1365-201X. ; 171:4, s. 419-425
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- We recently demonstrated that intravenous (i.v.) injection of the iron-binding protein lactoferrin (Lf) followed by antilactoferrin (aLf) antibodies or iron-saturated Lf alone increased albumin extravasation in vivo in several tissues including skin. Increased driving pressure for blood-tissue exchange or direct effects of Lf on the endothelial barrier are possible mechanisms. We therefore, firstly, measured interstitial fluid pressure (Pif) in dermis of rats given 1 mg Lf i.v. followed 30 min later by aLf or saline and circulatory arrest 1 or 5 min thereafter and compared with controls. Secondly, transmonolayer passage of Evans blue labelled albumin (EB-albumin) was evaluated in porcine pulmonary artery endothelial cells exposed to iron-free or iron-saturated Lf (both 100 ╡g mL-1) in the absence and presence of 0.5 mM hydrogen peroxide. Pif increased significantly at 11-30 min following Lf to +2.1 ▒ 0.3 and +1.7 ▒ 0.2 mmHg at 11-20 and 21-30 min, respectively, compared with +0.1 ▒ 0.2 mmHg before Lf (P < 0.05, n = 25). Endothelial transmonolayer passage of EB-albumin during 3 h was not affected by iron-free or iron-saturated Lf neither in the absence nor presence of hydrogen peroxide that increased passage 3.5 times compared with controls. In conclusion, Lf-induced increase in albumin extravasation in rat skin is not explained by changes in Pif (because Lf raised Pif significantly) or direct effects of Lf on the endothelial barrier.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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