Sökning: id:"swepub:oai:DiVA.org:liu-39758" >
Reduced CCR4, inter...
Reduced CCR4, interleukin-13 and GATA-3 up-regulation in response to type 2 cytokines of cord blood T lymphocytes in infants at genetic risk of type 1 diabetes
-
- Luopajärvi, Kristiina (författare)
- Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland
-
- Skarsvik, Susanne (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
-
- Ilonen, Jorma (författare)
- Department of Clinical Microbiology, University of Kuopio, Kuopio and Immunogenetics Laboratory, University of Turku, Turku, Finland
-
visa fler...
-
- Åkerblom, Hans (författare)
- Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland
-
- Vaarala, Outi, 1962- (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
-
visa färre...
-
(creator_code:org_t)
- Wiley, 2007
- 2007
- Engelska.
-
Ingår i: Immunology. - : Wiley. - 0019-2805 .- 1365-2567. ; 121:2, s. 189-196
- Relaterad länk:
-
https://www.ncbi.nlm...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Aberrancies in T-cell polarization including expression of chemokine receptors have been reported in human leucocyte antigen (HLA) class II associated autoimmune diseases, such as type 1 diabetes (T1D) and rheumatoid arthritis. We asked whether these aberrancies are present at birth in newborn infants carrying the HLA risk haplotypes for T1D. Sixty-seven cord blood (CB) samples from infants were screened for T1D-associated HLA risk genotypes (HLA-DR4-DQ8 and/or DR3-DQ2 without protective alleles). CB lymphocytes were stimulated with phytohaemagglutinin in type 1 (interleukin (IL)-12, anti-IL4) or type 2 (IL-4, anti-IL12) cytokine environment for 6 days. The expression of chemokine and cytokine receptors on T cells was determined by flow cytometry, secretion of cytokines was analysed with enzyme-linked immunosorbent assay, and transcription factors were analysed using real-time reverse transcriptase–polymerase chain reaction. After culture of CB lymphocytes in type 2 cytokine environment newborn infants carrying DR4-DQ8 haplotype (n = 18) showed reduced percentage of CD4 T cells expressing CCR4 (P = 0·009) and the level of CCR4 mRNA was decreased (P = 0·008). In addition, lower secretion of IL-13 and expression of GATA-3 in CB lymphocytes cultured in type 2 cytokine environment were found in the infants with DR4-DQ8 haplotype (P = 0·020 and P = 0·004, respectively) in comparison to newborn infants without DR4-DQ8 and DR3-DQ2 haplotypes (n = 37). Poor in vitro induction of type 2 immune responses in newborn infants with DR4-DQ8 haplotype suggests that the HLA genotype associated with risk of autoimmunity may affect the T cell polarization already at birth, which in turn may contribute to the risk for autoimmunity later in life.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas