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Protein microarray ...
Protein microarray analysis as a tool for monitoring cellular autoreactivity in type 1 diabetes patients and their relatives
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Vrabelova, Z (författare)
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Kolouskova, S (författare)
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Böhmova, K (författare)
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- Faresjö, Maria, 1971- (författare)
- Linköpings universitet,Hälsouniversitetet,Pediatrik,Hälsouniversitetet i Linköping
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Sumnik, Z (författare)
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Pechova, M (författare)
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Kverka, M (författare)
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Chudoba, D (författare)
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Zacharovova, K (författare)
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Stadlerova, G (författare)
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Pithova, P (författare)
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Hladikova, M (författare)
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Stechova, K (författare)
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(creator_code:org_t)
- Hindawi Limited, 2007
- 2007
- Engelska.
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Ingår i: Pediatric Diabetes. - : Hindawi Limited. - 1399-543X .- 1399-5448. ; 8:5, s. 252-260
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: Autoreactive T cells have a crucial role in type 1 diabetes (T1D) pathogenesis.: Objectives: The aim of our study was to monitor the in vitro production of cytokines by peripheral blood mononuclear cells (PBMCs) after stimulation with diabetogenic autoantigens. Subjects: Ten T1D patients (tested at the time of diagnosis and 6 and 12 months later), 10 first-degree relatives of the T1D patients, and 10 controls underwent the study. Methods: PBMCs were stimulated with glutamic acid decarboxylase 65 (GAD65) amino acids (a.a.) 247-279, 509-528, and 524-543, proinsulin a.a. 9-23, and tyrosine phosphatase (islet antigen-2)/R2 a.a. 853-872. Interleukin (IL)-2, IL-4, IL-5, IL-6, IL-10, IL-13, interferon (IFN)-γ, tumor necrosis factor β, transforming growth factor β1, and granulocyte colony-stimulating factor (GCSF) were analyzed by protein microarray. Results: Differences in cytokine(s) poststimulatory and mainly in basal production were observed in all groups. The most prominent findings were in controls, the higher basal levels of IL-2, IL-4, IL-5, IL-13, and GCSF were observed when compared with relatives (p < 0.05, for all). After stimulation in controls, there was a significant decrease in IL-2, IL-13, GCSF, and IFN-γ (p < 0.05, for all). The group of relatives was the most variable in poststimulatory production. A strong correlation between cytokines production was found but groups differed in this aspect. Conclusion: By multiplex analysis, it may be possible, for example, to define the risk immunological response pattern among relatives or to monitor the immune response in patients on immune modulation therapy. © 2007 The Authors Journal compilation © 2007 Blackwell Munksgaard.
Nyckelord
- autoantigen
- autoreactive T cells
- cytokine
- protein microaray
- T1D
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Vrabelova, Z
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Kolouskova, S
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Böhmova, K
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Faresjö, Maria, ...
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Sumnik, Z
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Pechova, M
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visa fler...
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Kverka, M
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Chudoba, D
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Zacharovova, K
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Stadlerova, G
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Pithova, P
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Hladikova, M
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Stechova, K
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visa färre...
- Artiklar i publikationen
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Pediatric Diabet ...
- Av lärosätet
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Linköpings universitet
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Jönköping University