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Probiotics enhance ...
Probiotics enhance pancreatic glutathione biosynthesis and reduce oxidative stress in experimental acute pancreatitis
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- Lutgendorff, Femke, 1981- (författare)
- Linköpings universitet,Hälsouniversitetet,Institutionen för klinisk och experimentell medicin
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- Trulsson, Lena, 1950- (författare)
- Linköpings universitet,Hälsouniversitetet,Kirurgi
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- van Minnen, L. Paul (författare)
- Department of Surgery University Medical Center, Utrecht, The Netherlands
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- Rijkers, Ger T. (författare)
- Department of Surgery University Medical Center, Utrecht, The Netherlands
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- Timmerman, Harro M. (författare)
- Department of Surgery University Medical Center, Utrecht, The Netherlands
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- Franzén, Lennart E. (författare)
- 3Department of Pathology and Cytology Aleris Medilab, Täby
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- Gooszen, Hein G. (författare)
- Department of Surgery University Medical Center, Utrecht, The Netherlands
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- Akkermans, Louis M. A. (författare)
- Department of Surgery University Medical Center, Utrecht, The Netherlands
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- Söderholm, Johan D, 1958- (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Kirurgi,Kirurgiska kliniken i Östergötland med verksamhet i Linköping, Norrköping och Motala
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- Sandström, Per, 1965- (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Kirurgi,Kirurgiska kliniken i Östergötland med verksamhet i Linköping, Norrköping och Motala
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(creator_code:org_t)
- American Physiological Society, 2008
- 2008
- Engelska.
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Ingår i: American Journal of Physiology - Gastrointestinal and Liver Physiology. - : American Physiological Society. - 0193-1857 .- 1522-1547. ; 295:5
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Factors determining severity of acute pancreatitis (AP) are poorly understood. Oxidative stress causes acinar cell injury and contributes to the severity, whereas prophylactic probiotics ameliorate experimental pancreatitis. Our objective was to study how probiotics affect oxidative stress, inflammation, and acinar cell injury during the early phase of AP. Fifty-three male Sprague-Dawley rats were randomly allocated into groups: 1) control, 2) sham procedure, 3) AP with no treatment, 4) AP with probiotics, and 5) AP with placebo. AP was induced under general anesthesia by intraductal glycodeoxycholate infusion (15 mM) and intravenous cerulein (5 μg·kg-1·h-1, for 6 h). Daily probiotics or placebo were administered intragastrically, starting 5 days prior to AP. After cerulein infusion, pancreas samples were collected for analysis including lipid peroxidation, glutathione, glutamate-cysteine-ligase activity, histological grading of pancreatic injury, and NF-κB activation. The severity of pancreatic injury correlated to oxidative damage (r = 0.9) and was ameliorated by probiotics (1.5 vs. placebo 5.5, P = 0.014). AP-induced NF-κB activation was reduced by probiotics (0.20 vs. placebo 0.53 OD 450nm/mg nuclear protein, P < 0.001). Probiotics attenuated AP-induced lipid peroxidation (0.25 vs. placebo 0.51 pmol malondialdehyde/mg protein, P < 0.001). Not only was AP-induced glutathione depletion prevented (8.81 vs. placebo 4.1 μmol/mg protein, P < 0.001), probiotic pretreatment even increased glutathione compared with sham rats (8.81 vs. sham 6.18 μmol/mg protein, P < 0.001). Biosynthesis of glutathione (glutamate-cysteine-ligase activity) was enhanced in probiotic-pretreated animals. Probiotics enhanced the biosynthesis of glutathione, which may have reduced activation of inflammation and acinar cell injury and ameliorated experimental AP, via a reduction in oxidative stress. Copyright © 2008 the American Physiological Society.
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- MEDICIN
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Lutgendorff, Fem ...
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van Minnen, L. P ...
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Rijkers, Ger T.
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Timmerman, Harro ...
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Franzén, Lennart ...
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Gooszen, Hein G.
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