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Silane-dextran chem...
Silane-dextran chemistry on lateral flow polymer chips for immunoassays
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- Jonsson, C. (författare)
- Jönsson, C., Åmic AB, Uppsala, Sweden, Biomedical Diagnostics Institute, NCSR Building, Dublin City University, Glasnevin Dublin 9, Ireland
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- Aronsson, M. (författare)
- Åmic AB, Uppsala, Sweden
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- Rundstrom, G. (författare)
- Rundström, G., Åmic AB, Uppsala, Sweden
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- Pettersson, C. (författare)
- Åmic AB, Uppsala, Sweden
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- Mendel-Hartvig, I. (författare)
- Åmic AB, Uppsala, Sweden
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- Bakker, J. (författare)
- Åmic AB, Uppsala, Sweden, Biomedical Diagnostics Institute, NCSR Building, Dublin City University, Glasnevin Dublin 9, Ireland
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- Martinsson, Erik (författare)
- Linköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan
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- Liedberg, Bo (författare)
- Linköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan
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- MacCraith, B. (författare)
- Biomedical Diagnostics Institute, NCSR Building, Dublin City University, Glasnevin Dublin 9, Ireland
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- Ohman, O. (författare)
- Öhman, O., Åmic AB, Uppsala, Sweden
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- Melin, J. (författare)
- Åmic AB, Uppsala, Sweden, Biomedical Diagnostics Institute, NCSR Building, Dublin City University, Glasnevin Dublin 9, Ireland
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Jönsson, C, Åmic AB, Uppsala, Sweden, Biomedical Diagnostics Institute, NCSR Building, Dublin City University, Glasnevin Dublin 9, Ireland Åmic AB, Uppsala, Sweden (creator_code:org_t)
- Royal Society of Chemistry (RSC), 2008
- 2008
- Engelska.
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Ingår i: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 8:7, s. 1191-1197
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The prognosis for patients suffering from cardiovascular and many other diseases can be substantially improved if diagnosed at an early stage. High performance diagnostic testing using disposable microfluidic chips can provide a platform for realizing this vision. Åmic AB (Uppsala, Sweden) has developed a new microfluidic test chip for sandwich immunoassays fabricated by injection molding of the cycloolefin-copolymer Zeonor™. A highly ordered array of micropillars within the fluidic chip distributes the sample solution by capillary action. Since wetting of the pillar array surface is the only driving force for liquid distribution precise control of the surface chemistry is crucial. In this work we demonstrate a novel protocol for surface hydrophilization and antibody immobilization on cycloolefin-copolymer test chips, based on direct silanisation of the thermoplastic substrate. Dextran is subsequently covalently coupled to amino groups, thus providing a coating with a low contact angle suitable for antibody immobilization. The contact angle of dextran coated chips is stable for at least two months, which enables production of large batches that can be stored for extended periods of time. We demonstrate the utility of the presented platform and surface chemistry in a C-reactive protein assay with a detection limit of 2.6 ng ml-1, a dynamic range of 102 and a coefficient of variance of 15%. © The Royal Society of Chemistry.
Nyckelord
- TECHNOLOGY
- TEKNIKVETENSKAP
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- art (ämneskategori)
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Jonsson, C.
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Aronsson, M.
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Rundstrom, G.
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Pettersson, C.
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Mendel-Hartvig, ...
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Bakker, J.
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visa fler...
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Martinsson, Erik
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Liedberg, Bo
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MacCraith, B.
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Ohman, O.
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Melin, J.
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visa färre...
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Lab on a Chip
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Linköpings universitet