Sökning: id:"swepub:oai:DiVA.org:liu-47265" >
Protein adsorption ...
Protein adsorption to oligo(ethylene glycol) self-assembled monolayers : Experiments with fibrinogen, heparinized plasma, and serum
-
- Benesch, Johan (författare)
- Linköpings universitet,Tekniska högskolan,Institutionen för fysik, kemi och biologi
-
- Svedhem, Sofia (författare)
- Linköpings universitet,Kemi,Tekniska högskolan
-
- Svensson, Stefan (författare)
- Linköpings universitet,Tekniska högskolan,Organisk Kemi
-
visa fler...
-
- Valiokas, Ramunas (författare)
- Linköpings universitet,Tekniska högskolan,Institutionen för fysik, kemi och biologi
-
- Liedberg, Bo (författare)
- Linköpings universitet,Tekniska högskolan,Sensorvetenskap och Molekylfysik
-
- Tengvall, Pentti (författare)
- Linköpings universitet,Tekniska högskolan,Tillämpad Fysik
-
visa färre...
-
(creator_code:org_t)
- Taylor & Francis, 2001
- 2001
- Engelska.
-
Ingår i: Journal of Biomaterials Science. Polymer Edition. - : Taylor & Francis. - 0920-5063 .- 1568-5624. ; 12:6, s. 581-597
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Low protein adsorption is believed advantageous for blood-contacting materials and ethylene glycols (EG)-based polymeric compounds are often attached to surfaces for this purpose. In the present study, the adsorption of fibrinogen, serum, and plasma were studied by ellipsometry on a series of well-defined oligo(EG) terminated alkane-thiols self-assembled on gold. The layers were prepared with compounds of the general structure HS-(CH2)15-CONH-EGn, where n = 2, 4, and 6. Methoxy-terminated tri(EG) undecanethiol and hydroxyl-terminated hexadecanethiol self-assembled monolayers (SAMs) were used as references. The results clearly demonstrate that the adsorption depends on the experimental conditions with small amounts of fibrinogen adsorbing from a single protein solution, but larger amounts of proteins from serum and plasma. The adsorption of fibrinogen and blood plasma decreased with an increasing number of EG repeats and was temperature-dependent. Significantly less serum adsorbed to methoxy tri(EG) than to hexa(EG) and more proteins remained on the latter surface after incubation in a sodium dodecyl sulfate (SDS) solution, indicating a looser protein binding to the methoxy-terminated surface. All surfaces adsorbed complement factor 3(C3) from serum and plasma, although no surface-mediated complement activation was observed. The present study points to the importance of a careful choice of the protein model system before general statements regarding the protein repellant properties of potential surfaces can be made.
Nyckelord
- Complement
- Fibrinogen
- Heparinized plasma
- Oligo(ethylene glycol)
- Protein adsorption
- Self-assembled monolayers
- Serum
- TECHNOLOGY
- TEKNIKVETENSKAP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas