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Enhanced expression...
Enhanced expression of cyclooxygenase-2 and nuclear beta-catenin are related to mutations in the APC gene in human colorectal cancer
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- Dimberg, Jan (författare)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
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- Hugander, A (författare)
- Univ Jonkoping, Coll Hlth Sci, Dept Nat Sci & Biomed, S-55111 Jonkoping, Sweden Fac Hlth Sci, Div Cell Biol, Dept Biomed & Surg, Linkoping, Sweden Ryhov Cty Hosp, Dept Surg, Jonkoping, Sweden Karolinska Hosp, Karolinska Inst, Ctr Mol Med, S-10401 Stockholm, Sweden
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- Sirsjo, A (författare)
- Univ Jonkoping, Coll Hlth Sci, Dept Nat Sci & Biomed, S-55111 Jonkoping, Sweden Fac Hlth Sci, Div Cell Biol, Dept Biomed & Surg, Linkoping, Sweden Ryhov Cty Hosp, Dept Surg, Jonkoping, Sweden Karolinska Hosp, Karolinska Inst, Ctr Mol Med, S-10401 Stockholm, Sweden
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visa fler...
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- Söderkvist, Peter (författare)
- Linköpings universitet,Hälsouniversitetet,Cellbiologi
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visa färre...
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: Anticancer Research. - 0250-7005 .- 1791-7530. ; 21:2A, s. 911-915
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Mutational inactivation of the human tumour suppressor gene adenomatous polyposis coli (APC) results in constitutive activation of beta -catenin/T cell factor-4 (Tcf-4) mediated transcription of target genes. Up-regulation of cyclooxygenase-2 (COX-2) protein is frequently found in human colorectal cancer (CRC). We analysed 38 CRC for mutations in APC and beta -catenin and found an association between APC mutations and elevated COX-2 levels. Furthermore, APC mutations were predominantly observed in tumour tissues from the rectum compared to tumours of colonic origin. Western blot analysis revealed that nuclear beta -catenin levels were generally higher in tumours with APC mutations compared to tumours with wild type APC. However, there was also a higher level of nuclear beta -catenin in tumour compared to normal tissue, hut nuclear Tcf-4 protein was constitutively expressed in tumour and normal tissue and showed no differences. An identified putative Tcf-4 binding element in the COX-2 promoter may partly explain the enhanced level of COX-2 and support the idea that COX-2 may be a downstream target of the APC/beta -catenin/Tcf-4 pathway.
Nyckelord
- cyclooxygenase-2
- beta-catenin
- Tcf-4
- APC
- colorectal cancer
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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