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D variants at the R...
D variants at the RhD vestibule in the weak D type 4 and Eurasian D clusters
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- Flegel, Willy A (författare)
- University Hospital, Ulm
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von Zabern, Inge (författare)
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Doescher, Andrea (författare)
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Wagner, Franz F (författare)
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Strathmann, Klaus P (författare)
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Geisen, Christof (författare)
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- Plafi, Miodrag (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Transfusionsmedicin,Hälsouniversitetet,Klinisk immunologi och transfusionsmedicin,Landstinget i Östergötland
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Pisacka, Martin (författare)
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Poole, Joyce (författare)
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Polin, Helene (författare)
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Gabriel, Christian (författare)
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Avent, Neil D (författare)
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(creator_code:org_t)
- Wiley, 2009
- 2009
- Engelska.
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Ingår i: Transfusion. - : Wiley. - 0041-1132 .- 1537-2995. ; 49:6, s. 1059-1069
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- BACKGROUND: One branch of the RHD phylogenetic tree is represented by the weak D type 4 cluster of alleles with F223V as the primordial amino acid substitution. F223V as well as a large number of further substitutions causing D variants are located at the extracellular RhD protein vestibule, which represents the entrance to the transmembraneous channel of the RhD protein. STUDY DESIGN AND METHODS: RHD and RHCE nucleotide sequences were determined from genomic DNA and cDNA. D epitope patterns were established with commercial monoclonal anti-D panels. RESULTS: The RHD alleles DOL-1 and DOL-2 had the two amino acid substitutions M170T (509T>C) and F223V (667T>G) in common. DOL-2 harbored the additional substitution L378V (1132C>G). Both alleles were observed in Africans and are probably evolutionary related. DMI carried M170I (510G>A), which differed from the DOL-typical substitution. DFW and DFL harbored the substitutions H166P (497A>C) and Y165C (494A>G). The antigen densities of DOL-1, DFL, and DFW were only moderately reduced. CONCLUSION: DOL-1 and DOL-2 belong to the weak D type 4 cluster of RHD alleles. Together with DMI, DFL, and DFW they represent D variants with amino acid substitutions located at extracellular loops 3 or 4 lining the RhD protein vestibule. These substitutions were of minor influence on antigen density while adjacent substitutions in the transmembraneous section caused weak D antigen expression. All these D variants were partial D and alloanti-D immunizations have been observed in DOL-1, DMI, and DFL carriers. The substitution at position 170 causes partial D although located deep in the vestibule.
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- MEDICINE
- MEDICIN
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- art (ämneskategori)
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Flegel, Willy A
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von Zabern, Inge
-
Doescher, Andrea
-
Wagner, Franz F
-
Strathmann, Klau ...
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Geisen, Christof
-
visa fler...
-
Plafi, Miodrag
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Pisacka, Martin
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Poole, Joyce
-
Polin, Helene
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Gabriel, Christi ...
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Avent, Neil D
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visa färre...
- Artiklar i publikationen
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Transfusion
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Linköpings universitet