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Stereoselective det...
Stereoselective determination of venlafaxine and its three demethylated metabolites in human plasma and whole blood by liquid chromatography with electrospray tandem mass spectrometric detection and solid phase extraction
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- Kingbäck, Maria (författare)
- Linköpings universitet,Klinisk farmakologi,Hälsouniversitetet
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- Josefsson, Martin (författare)
- Linköpings universitet,Institutionen för fysik, kemi och biologi,Tekniska högskolan,Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Artillerigatan 12, Linköping, Sweden
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- Karlsson, Louise (författare)
- Linköpings universitet,Klinisk farmakologi,Hälsouniversitetet
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- Ahlner, Johan (författare)
- Linköpings universitet,Klinisk farmakologi,Hälsouniversitetet,Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Artillerigatan 12, Linköping, Sweden
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- Bengtsson, Finn (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Klinisk farmakologi,Hälsouniversitetet
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- Kugelberg, Fredrik C (författare)
- Linköpings universitet,Klinisk farmakologi,Hälsouniversitetet,Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Artillerigatan 12, Linköping, Sweden
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- Carlsson, Björn (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Klinisk farmakologi,Hälsouniversitetet
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(creator_code:org_t)
- Elsevier BV, 2010
- 2010
- Engelska.
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Ingår i: Journal of Pharmaceutical and Biomedical Analysis. - : Elsevier BV. - 0731-7085 .- 1873-264X. ; 53:3, s. 583-590
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A stereoselective method is described for simultaneous determination of the S- and R-enantiomers of venlafaxine and its three demethylated metabolites in human plasma and whole blood samples. This validated method involved LC/MS/MS with positive electrospray ionization and solid phase extraction. Chromatographic separation was performed on a 250 mm x 2.1mm Chirobiotic V column with a total run time of 35 min. In plasma, calibration curves were in the range of 1-1000 nM for the S- and R-enantiomers of venlafaxine and O-desmethylvenlafaxine, and 0.5-500 nM for N-desmethylvenlafaxine and N,O-didesmethylvenlafaxine. In whole blood the corresponding concentrations were 10-4000 and 5-2000 nM, respectively. The intra-day precision was <6.3% and the inter-day precision was <9.9% for plasma and <15% and <19% for whole blood. LLOQ ranged between 0.25 and 0.5 nM. No ion suppression/enhancement or other matrix effects were observed. The method was successfully applied for determination of venlafaxine and its metabolites in plasma from patients and whole blood samples from forensic autopsy cases.
Nyckelord
- Venlafaxine; Enantiomers; LC–ESI-MS/MS; Metabolites; Human
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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