Amperometric tetrathiafulvalene-mediated lactate electrode using lactate oxidase absorbed on carbon foil

• The features of a new sensor for determining l-lactate are reported. The enzyme lactate oxidase and the mediator, tetrathiafulvalene (TTF), are absorbed on carbon foil disks previously bonded onto the ends of glass tubes. Linear calibration graphs were obtained in the range 10−4−10−3 M with physiological phosphate buffer (pH 7.35) and at 30°C with a response time of a few seconds. Calibration graphs in the range 10−3−10−2 M were also obtained and the difference in response times between these two ranges were investigated. The results are promising for assembling disposable lactate sensors for in vitro or for in or ex vivo measurements.

Nyckelord

Lactate

Enzyme electrode

Tetrathiafulvalene mediator

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