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Roles of Ca2+ and F...
Roles of Ca2+ and F-actin in intracellular aggregation of Chlamydia trachomatis in eucaryotic cells
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- Majeed, Meytham (författare)
- Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet
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- Gustafsson, Mikael (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Kihlström, Erik (författare)
- Department of clinical microbiology, Örebro medical center hospital, Örebro, Sweden
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- Stendahl, Olle (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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(creator_code:org_t)
- American Society for Microbiology, 1993
- 1993
- Engelska.
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Ingår i: Infection and Immunity. - : American Society for Microbiology. - 0019-9567 .- 1098-5522. ; 61:4, s. 1406-1414
- Relaterad länk:
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http://iai.asm.org/c...
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https://urn.kb.se/re...
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Abstract
Ämnesord
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- The effect of intracellular free Ca2+ ([Ca2+]i) on the intracellular aggregation of Chlamydia trachomatis serovars L2 and E in McCoy and HeLa cells is investigated. Loading the cells with the Ca2+ chelator MAPT/AM (1,2-bis-5-methyl-amino-phenoxylethane-N,N-n'-tetra-acetoxymethyl acetate), thereby decreasing the [Ca2+]i from 67 to 19 nM, decreased the number of cells with a local aggregation of chlamydiae in a dose-dependent manner. Neither the attachment nor the uptake of elementary bodies (EBs) was, however, affected after depletion of Ca2+ from the cells. There was no significant difference in the level of measured [Ca2+]i between infected and uninfected cells. Reducing the [Ca2+]i also significantly inhibited chlamydial inclusion formation. Differences in the organization of the actin filament network were observed in response to [Ca2+]i depletion. In Ca(2+)-depleted cells, where few EB aggregates were formed, few local accumulations of F-actin were observed in the cytosol. These results suggest that the aggregation of EBs in eucaryotic cells requires a normal homeostasis of intracellular Ca2+. By affecting F-actin reorganization and putatively certain Ca(2+)-binding proteins, [Ca2+]i plays a vital role in the infectious process of chlamydiae.
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