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Simultaneous Measur...
Simultaneous Measurement of 11 Antibiotics for Use in the Intensive Care Unit by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
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- Woksepp, Hanna (författare)
- Linnéuniversitetet,Institutionen för medicin och optometri (MEO),Kalmar County Hospital, Sweden
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- Karlsson, Louise (författare)
- Linköping University, Sweden
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- Arlemalm, Andreas (författare)
- Linköping University, Sweden
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- Hallgren, Anita (författare)
- Linköping University, Sweden
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- Schön, Thomas (författare)
- Linköping University Hospital, Sweden;Linköping University, Sweden;Kalmar County Hospital, Sweden
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- Carlsson, Björn (författare)
- Linköping University, Sweden
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(creator_code:org_t)
- Lippincott Williams & Wilkins, 2022
- 2022
- Engelska.
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Ingår i: Therapeutic Drug Monitoring. - : Lippincott Williams & Wilkins. - 0163-4356 .- 1536-3694. ; 44:2, s. 308-318
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: Recent studies indicate that a high proportion of patients in the intensive care unit fail to attain adequate antibiotic levels. Thus, there is a need to monitor the antibiotic concentration to ensure effective treatment. In this article, the authors aimed to develop an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous quantification of antimicrobials to assess individualized therapeutic drug monitoring. Methods: A UHPLC-MS/MS method with 11 antibiotics (ciprofloxacin, moxifloxacin, benzylpenicillin, levofloxacin, linezolid, rifampicin, meropenem, cloxacillin, cefotaxime, clindamycin, and piperacillin) was developed. Chromatographic separation was performed using a Kinetex Biphenyl reversed-phase column, with gradient elution using 0.1% formic acid and methanol with 0.1% formic acid. Sample preparation was performed using methanol protein precipitation. The total run time was 5 minutes. Results: For all analytes, the interassay inaccuracies for calibrators were <= 5%. The interday inaccuracies for the quality controls (QCs) were <= 5% for all analytes. The interassay precision for calibration standards ranged between 1.42% and 6.11%. The interassay imprecision for QCs of all antibiotics and concentrations ranged between 3.60% and 16.1%. Interassay inaccuracy and imprecision for the QCs and calibration standards were <= 15% for all drugs, except benzylpenicillin. Conclusions: A rapid UHPLC-MS/MS method was developed for the simultaneous quantification of 11 different antibiotics. Minimal sample preparation was required to ensure a rapid turnaround time. The method was applied to clinical samples collected from 4 intensive care units.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- UHPLC-MS
- MS
- antibiotics
- therapeutic drug monitoring
- critically ill patients
- clinical application
- Pharmacology
- Farmakologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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