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De Novo-Designed En...
De Novo-Designed Enzymes as Small-Molecule-Regulated Fluorescence Imaging Tags and Fluorescent Reporters
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- Liu, Yu (författare)
- The Scripps Research Institute, USA
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- Zhang, Xin (författare)
- The Scripps Research Institute, USA
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- Tan, Yun Lei (författare)
- The Scripps Research Institute, USA
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- Bhabha, Gira (författare)
- University of California, USA
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- Ekiert, Damian C (författare)
- University of California, USA
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- Kipnis, Yakov (författare)
- University of Washington, USA
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- Bjelic, Sinisa (författare)
- University of Washington, USA
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- Baker, David (författare)
- University of Washington, USA
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- Kelly, Jeffery W (författare)
- The Scripps Research Institute, USA
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(creator_code:org_t)
- 2014-09-11
- 2014
- Engelska.
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 136:38, s. 13102-13105
- Relaterad länk:
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https://pubs.acs.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Enzyme-based tags attached to a protein-of-interest (POI) that react with a small molecule, rendering the conjugate fluorescent, are very useful for studying the POI in living cells. These tags are typically based on endogenous enzymes, so protein engineering is required to ensure that the small-molecule probe does not react with the endogenous enzyme in the cell of interest. Here we demonstrate that de novo-designed enzymes can be used as tags to attach to POIs. The inherent bioorthogonality of the de novo-designed enzyme-small-molecule probe reaction circumvents the need for protein engineering, since these enzyme activities are not present in living organisms. Herein, we transform a family of de novo-designed retroaldolases into variable-molecular-weight tags exhibiting fluorescence imaging, reporter, and electrophoresis applications that are regulated by tailored, reactive small-molecule fluorophores.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Biokemi
- Biochemistry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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