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Phenylpropanoid Met...
Phenylpropanoid Metabolism in Suspension Cultures of Vanilla planifolia. Andr. II. Effects of Precursor Feeding and Metabolic Inhibitors
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Funk, C (författare)
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- Brodelius, Peter (författare)
- Department of Plant Biochemistry, University of Lund
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(creator_code:org_t)
- 1990
- 1990
- Engelska.
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Ingår i: Plant Physiology. - 0032-0889 .- 1532-2548. ; 94, s. 95-101
- Relaterad länk:
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http://www.plantphys...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Feeding of cinnamic acid and ferulic acid to non-treated andchitosan-treated cell suspension cultures of Vanilla planifoIiaresulted in the formation of trace amounts of p-hydroxy benzoicacid (5.2 micrograms per gram fresh weight of cells) and vanillicacid (6.4 micrograms per gram fresh weight of cells), respectively.Addition of a 4-hydroxycinnamate: CoA-ligase inhibitor, 3,4-(methylenedioxy)-cinnamic acid (MDCA), resulted in a reducedbiosynthesis of ligneous material with a simultaneous significantincreased vanillic acid formation (around 75 micrograms per gramfresh weight of cells). A K, of 100 micromolar for 4-hydroxycinnamate:CoA-ligase in a crude preparation was estimated for thisinhibitor. It is suggested that the conversion of cinnamic acidsinto benzoic acids does not involve cinnamoyl CoA esters asintermediates. Feeding of 14C-cinnamic acid and 14C-ferulic acidto cells treated with MDCA indicate that cinnamic acid, but notferulic acid, is a precursor of vanillic acid in these cultivated cellsof V. planifolia.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Biochemistry
- Biokemi
- Biochemistry
- Biokemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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