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Evaluation of a Nov...
Evaluation of a Novel Immunoassay for Quantification of C1q for Clinical Diagnostic Use
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- Sandholm, Kerstin (författare)
- Linnaeus University,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Linnaeus Ctr Biomat Chem, BMC,Linnaeus Univ, Linnaeus Ctr Biomat Chem, Kalmar, Sweden
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- Persson, Barbro (författare)
- Uppsala universitet,Uppsala University,Uppsala University, Sweden,Klinisk immunologi
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- Skattum, Lillemor (författare)
- Lund University,Lunds universitet,Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Microbiology, Immunology and Glycobiology - MIG,Department of Laboratory Medicine,Faculty of Medicine,Lund University, Sweden,Lund Univ, Sect Microbiol, Dept Lab Med Immunol & Glycobiol, Lund, Sweden
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- Eggertsen, Gösta (författare)
- Karolinska Institutet,Karolinska Institutet, Sweden;Karolinska University Hospital, Sweden,Karolinska Inst, Dept Lab Med, Stockholm, Sweden;Karolinska Univ Lab, Clin Chem, Stockholm, Sweden
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- Nyman, Dag (författare)
- Aland Cent Hosp, Aland Borrelia Grp, Mariehamn, Finland,Åland Central Hospital
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- Gunnarsson, Iva (författare)
- Karolinska Institutet,Karolinska Institutet, Sweden;Karolinska University Hospital, Sweden,Karolinska Inst, Karolinska Univ Hosp, Dept Med, Rheumatol Unit, Stockholm, Sweden
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- Svenungson, Elisabet (författare)
- Karolinska Institutet,Karolinska Institutet, Sweden;Karolinska University Hospital, Sweden,Karolinska Inst, Karolinska Univ Hosp, Dept Med, Rheumatol Unit, Stockholm, Sweden
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- Nilsson, Bo (författare)
- Uppsala universitet,Uppsala University,Uppsala University, Sweden,Klinisk immunologi
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- Nilsson Ekdahl, Kristina (författare)
- Uppsala universitet,Linnaeus University,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Uppsala University, Sweden,Linnaeus Ctr Biomat Chem, BMC,Klinisk immunologi,Linnaeus Univ, Linnaeus Ctr Biomat Chem, Kalmar, Sweden
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(creator_code:org_t)
- 2019-01-25
- 2019
- Engelska.
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Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 10
- Relaterad länk:
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https://doi.org/10.3...
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https://lnu.diva-por... (primary) (Raw object)
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https://www.frontier...
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http://dx.doi.org/10... (free)
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://lup.lub.lu.s...
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http://kipublication...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Objectives: C1q is a valuable biomarker of disease activity in systemic lupus erythematosus (SLE). The "gold standard" assay, rocket immunoelectrophoresis (RIE), is time-consuming, and thus a shift to soluble immune precipitation techniques such as nephelometry has occurred. However, quantification of C1q with these techniques has been questioned as a result of the antibody binding properties of C1q. In the present work, we have compared results using various techniques (RIE, nephelometry, and ELISA) and have developed and validated a new magnetic bead-based sandwich immunoassay (MBSI). Methods: C1q was quantified by nephelometry and the new sandwich immunoassay in 45 serum samples analyzed using RIE. C1q was also assessed in plasma using RIE and sandwich immunoassay in samples from SLE patients with nephritis (n = 69), SLE patients without nephritis (n = 310) as classified by BILAG score, and matched controls (n = 322). In addition, cerebrospinal fluid (CSF) samples from 31 patients, previously analyzed with ELISA, were also analyzed with the MBSI to test the behavior of this new assay in the lower detection range. Results: We found a strong correlation between the new MBSI, RIE, and ELISA, but not with nephelometry. The MBSI demonstrated lower levels of C1q in SLE patients than in matched controls (p < 0.0001), and patients with nephritis had lower levels than patients without nephritis (p < 0.01). Similarily, RIE showed significant differences between the patient groups (p < 0.0001). An association was also found between the levels of C1q and the SLE disease activity index (SLEDAI). Furthermore, there was good correlation between the values obtained by MBSI and ELISA, in both serum (r = 0.960) and CSF (r = 0.786), underscoring the ability of both techniques to measure low concentrations of C1q with high accuracy. Conclusion: The sandwich immunoassay correlated well with RIE, but soluble immune precipitation techniques, such as nephelometry, did not appear suitable alternatives, since C1q itself, and possibly anti-C1q antibodies, interfered with the measurements. The new sandwich immunoassay is therefore a good replacement for RIE in monitoring SLE disease activity.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- C1q
- immunoassays
- plasma
- CSF
- SLE
- nephritis
- Immunologi
- Immunology
- C1q
- CSF
- immunoassays
- nephritis
- plasma
- SLE
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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