Sökning: id:"swepub:oai:DiVA.org:lnu-80287" >
UCYN-A3, a newly ch...
UCYN-A3, a newly characterized open ocean sublineage of the symbiotic N2-fixing cyanobacterium Candidatus Atelocyanobacterium thalassa
-
- Cornejo-Castillo, Francisco M. (författare)
- Univ Calif Santa Cruz, USA;CSIC, Spain
-
- del Carmen Munoz-Marin, Maria (författare)
- Univ Calif Santa Cruz, USA;Univ Cordoba, Spain
-
- Turk-Kubo, Kendra A. (författare)
- Univ Calif Santa Cruz, USA
-
visa fler...
-
- Royo-Llonch, Marta (författare)
- CSIC, Spain
-
- Farnelid, Hanna, 1983- (författare)
- Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Univ Calif Santa Cruz, USA,Ctr Ecol & Evolut Microbial Model Syst EEMiS
-
- Acinas, Silvia G. (författare)
- CSIC, Spain
-
- Zehr, Jonathan P. (författare)
- Univ Calif Santa Cruz, USA
-
visa färre...
-
(creator_code:org_t)
- 2018-11-08
- 2019
- Engelska.
-
Ingår i: Environmental Microbiology. - : Wiley-Blackwell. - 1462-2912 .- 1462-2920. ; 21:1, s. 111-124
- Relaterad länk:
-
https://digital.csic...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The symbiotic unicellular cyanobacterium Candidatus Atelocyanobacterium thalassa (UCYN-A) is one of the most abundant and widespread nitrogen (N-2)-fixing cyanobacteria in the ocean. Although it remains uncultivated, multiple sublineages have been detected based on partial nitrogenase (nifH) gene sequences, including the four most commonly detected sublineages UCYN-A1, UCYN-A2, UCYN-A3 and UCYN-A4. However, very little is known about UCYN-A3 beyond the nifH sequences from nifH gene diversity surveys. In this study, single cell sorting, DNA sequencing, qPCR and CARD-FISH assays revealed discrepancies involving the identification of sublineages, which led to new information on the diversity of the UCYN-A symbiosis. 16S rRNA and nifH gene sequencing on single sorted cells allowed us to identify the 16S rRNA gene of the uncharacterized UCYN-A3 sublineage. We designed new CARD-FISH probes that allowed us to distinguish and observe UCYN-A2 in a coastal location (SIO Pier; San Diego) and UCYN-A3 in an open ocean location (Station ALOHA; Hawaii). Moreover, we reconstructed about 13% of the UCYN-A3 genome from Tara Oceans metagenomic data. Finally, our findings unveil the UCYN-A3 symbiosis in open ocean waters suggesting that the different UCYN-A sublineages are distributed along different size fractions of the plankton defined by the cell-size ranges of their prymnesiophyte hosts.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Ekologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Ecology (hsv//eng)
Nyckelord
- Mikrobiologi
- Microbiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas