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Regeneration of Ass...
Regeneration of Assembled, Molecular-Motor-Based Bionanodevices
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- Rahman, Mohammad A. (författare)
- Linnaeus University,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Lund University, Sweden
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- Reuther, Cordula (författare)
- Tech Univ Dresden, Germany;Max Planck Inst Mol Cell Biol & Genet, Germany,Dresden University of Technology,Max Planck Institute of Molecular Cell Biology and Genetics
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- Lindberg, Frida W. (författare)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
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- Mengoni, Martina (författare)
- Tech Univ Dresden, Germany;Max Planck Inst Mol Cell Biol & Genet, Germany,Max Planck Institute of Molecular Cell Biology and Genetics,Dresden University of Technology
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- Salhotra, Aseem (författare)
- Linnaeus University,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, 39182, Sweden; NanoLund, Lund University, Lund, 22100, Sweden
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- Heldt, Georg (författare)
- Fraunhofer Inst Elect Nano Syst, Germany,Fraunhofer Institute for Electronic Nanosystems (ENAS)
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- Linke, Heiner (författare)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
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- Diez, Stefan (författare)
- Tech Univ Dresden, Germany;Max Planck Inst Mol Cell Biol & Genet, Germany,Dresden University of Technology,Max Planck Institute of Molecular Cell Biology and Genetics
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- Månsson, Alf (författare)
- Linnaeus University,Lunds universitet,Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Avancerade material,Lund University, Sweden,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Other operations, LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- 2019-09-05
- 2019
- Engelska.
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Ingår i: Nano Letters. - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 19:10, s. 7155-7163
- Relaterad länk:
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https://doi.org/10.1...
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https://pubs.acs.org...
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http://dx.doi.org/10... (free)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- The guided gliding of cytoskeletal filaments, driven by biomolecular motors on nano/microstructured chips, enables novel applications in biosensing and biocomputation. However, expensive and time-consuming chip production hampers the developments. It is therefore important to establish protocols to regenerate the chips, preferably without the need to dismantle the assembled microfluidic devices which contain the structured chips. We here describe a novel method toward this end. Specifically, we use the small, nonselective proteolytic enzyme, proteinase K to cleave all surface-adsorbed proteins, including myosin and kinesin motors. Subsequently, we apply a detergent (5% SDS or 0.05% Triton X100) to remove the protein remnants. After this procedure, fresh motor proteins and filaments can be added for new experiments. Both, silanized glass surfaces for actin-myosin motility and pure glass surfaces for microtubule-kinesin motility were repeatedly regenerated using this approach. Moreover, we demonstrate the applicability of the method for the regeneration of nano/microstructured silicon-based chips with selectively functionalized areas for supporting or suppressing gliding motility for both motor systems. The results substantiate the versatility and a promising broad use of the method for regenerating a wide range of protein-based nano/microdevices.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
Nyckelord
- Nano/microdevice
- regeneration
- protein desorption
- molecular motor
- proteinase K
- detergent
- Biokemi
- Biochemistry
- detergent
- molecular motor
- Nano/microdevice
- protein desorption
- proteinase K
- regeneration
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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