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Microbial Productio...
Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties
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- Kanelli, Maria (författare)
- IndBioCat Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens
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- Mandic, Mina (författare)
- Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
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- Kalakona, Margarita (författare)
- IndBioCat Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athen
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- Vasilakos, Sozon (författare)
- Materials Industrial Research and Technology Center S.A., Athens
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- Kekos, Dimitris (författare)
- IndBioCat Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens
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- Nikodinovic-Runic, Jasmina (författare)
- Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
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- Topakas, Evangelos (författare)
- Luleå tekniska universitet,Kemiteknik,IndBioCat Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens
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(creator_code:org_t)
- 2018-07-10
- 2018
- Engelska.
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Ingår i: Frontiers in Microbiology. - : Frontiers Media S.A.. - 1664-302X. ; 9
- Relaterad länk:
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https://www.frontier...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- In the present study, crude bacterial extract containing violacein is investigated for the preparation of antimicrobial polyamide fabrics. The optimal culture conditions of Janthinobacterium lividum (JL) for maximum biomass and violacein production were found to be 25°C, pH 7.0, while the addition of ampicillin of 0.2 mg mL-1 in the small scale increased violacein production 1.3-fold. In scale-up trials, the addition of 1% (v/v) glycerol in a fed-batch bioreactor, resulted in fivefold extracted crude violacein increase with final concentration of 1.828 g L-1. Polyamide 6.6 fabrics were dyed following three different processes; through simultaneous fermentation and dyeing (SFD), by incubating the fabric in the sonicated bacterial culture after fermentation and by using cell-free extract containing violacein. Maximum color change (ΔE) and color strength (K/S) obtained for SFD fabrics were 74.81 and 22.01, respectively, while no alteration of fastness and staining of dye at acid and alkaline perspiration or at water was indicated. The dyed fabrics presented significant antifungal activity against Candida albicans, C. parapsilosis, and C. krusei, as well as antibacterial properties against Escherichia coli, Staphylococcus aureus, and the S. aureus MRSA. We have shown that J. lividum cultures can be successfully used for violacein production and for simultaneous dying of fabrics resulting in dyed fabrics with antimicrobial properties without utilization of organic solvents.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
Nyckelord
- Biokemisk processteknik
- Biochemical Process Engineering
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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