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Sökning: id:"swepub:oai:DiVA.org:ltu-83694" > Detection of SARS-C...

Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters

Kolarević, Stoimir (författare)
University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, Department of Hydroecology and Water Protection, Bulevar despota Stefana 142, 11000 Belgrade, Serbia
Micsinai, Adrienn (författare)
WESSLING Hungary Ltd., H-1045 Budapest, Anonymus str 6., Hungary
Szántó-Egész, Réka (författare)
Biomi Ltd., H-2100 Gödöllő, Szent-Györgyi Albert str 4., Hungary
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Lukács, Alena (författare)
Biomi Ltd., H-2100 Gödöllő, Szent-Györgyi Albert str 4., Hungary
Kračun-Kolarević, Margareta (författare)
University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, Department of Hydroecology and Water Protection, Bulevar despota Stefana 142, 11000 Belgrade, Serbia
Lundy, Lian (författare)
Luleå tekniska universitet,Arkitektur och vatten,Middlesex University, The Burroughs, London NW4 4BT, UK
Kirschner, Alexander K.T. (författare)
Medical University Vienna, Institute for Hygiene and Applied Immunology - Water Microbiology, Kinderspitalgasse 15, Vienna, Austria. Interuniversity Cooperation Center Water and Health (ICC), Austria. Karl Landsteiner University of Health Sciences, Division Water Quality & Health, Dr.-Karl-Dorrek-Straße 30, A-3500 Krems, Austria
Farnleitner, Andreas H. (författare)
Interuniversity Cooperation Center Water and Health (ICC), Austria. Karl Landsteiner University of Health Sciences, Division Water Quality & Health, Dr.-Karl-Dorrek-Straße 30, A-3500 Krems, Austria. Technische Universität Wien, Institute of Chemical, Environmental and Bioscience Engineering, Research Group for Environmental Microbiology and Molecular Diagnostics, Gumpendorferstraße 1a, A-1060 Vienna, Austria
Djukic, Aleksandar (författare)
Faculty of Civil Engineering, University of Belgrade, Bulevar kralja Aleksandra 73, 11000 Belgrade, Serbia
Čolić, Jasna (författare)
Jaroslav Černi Water Institute, Jaroslava Černog 80, 11226 Belgrade, Serbia
Nenin, Tanja (författare)
Jaroslav Černi Water Institute, Jaroslava Černog 80, 11226 Belgrade, Serbia
Sunjog, Karolina (författare)
University of Belgrade, Institute for Multidisciplinary Research, Kneza Višeslava 1, 11000 Belgrade, Serbia
Paunović, Momir (författare)
University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, Department of Hydroecology and Water Protection, Bulevar despota Stefana 142, 11000 Belgrade, Serbia
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University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, Department of Hydroecology and Water Protection, Bulevar despota Stefana 142, 11000 Belgrade, Serbia WESSLING Hungary Ltd, H-1045 Budapest, Anonymus str 6., Hungary (creator_code:org_t)
Elsevier, 2021
2021
Engelska.
Ingår i: Science of the Total Environment. - : Elsevier. - 0048-9697 .- 1879-1026. ; 783
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Samhällsbyggnadsteknik -- Vattenteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Civil Engineering -- Water Engineering (hsv//eng)

Nyckelord

SARS-CoV-2
Danube River
Faecal pollution
Untreated wastewaters
Serbia
VA-teknik
Urban Water Engineering
Centrumbildning - Centrum för dagvattenhantering (DRIZZLE)
Centre - Centre for Stormwater Management (DRIZZLE)

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