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LPS induces GROα ch...
LPS induces GROα chemokine production via NFκB in oral fibroblasts
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- Jönsson, Daniel (författare)
- Malmö högskola,Odontologiska fakulteten (OD)
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Amisten, Stefan (författare)
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Bratthall, Gunilla (författare)
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Holm, Anders (författare)
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Nilsson, Bengt-Olof (författare)
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(creator_code:org_t)
- 2009-05-09
- 2009
- Engelska.
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Ingår i: Inflammation Research. - : Springer. - 1023-3830 .- 1420-908X. ; 11:58, s. 791-796
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- OBJECTIVE AND DESIGN: Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GROalpha, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GROalpha by oral fibroblasts have not previously been presented. MATERIALS AND METHODS: GROalpha mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA. RESULTS: We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GROalpha in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GROalpha and GROalpha protein. The steroid hormone estrogen had no effect on LPS-induced GROalpha mRNA expression. Treatment with the glucocorticoid dexamethasone attenuated LPS-induced GROalpha production, and the NF-kappaB blocker MG 132 fully prevented LPS-induced GROalpha. CONCLUSIONS: Oral fibroblasts respond to LPS stimulation by increasing GROalpha production via the transcription factor NF-kappaB, suggesting that this mechanism may be involved in development of periodontal inflammation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Nyckelord
- Chemokines
- Dexamethasone
- Estrogen
- GROα
- PDL cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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