Sökning: id:"swepub:oai:DiVA.org:mau-42335" >
ApoE and ApoE Nasce...
ApoE and ApoE Nascent-Like HDL Particles at Model Cellular Membranes : Effect of Protein Isoform and Membrane Composition
-
- Waldie, Sarah (författare)
- Malmö University,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,Institut Laue-Langevin, Grenoble, France; Partnership for Structural Biology (PSB), Grenoble, France
-
- Sebastiani, Federica (författare)
- Malmö University,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
-
- Moulin, Martine (författare)
- Institut Laue-Langevin, Grenoble, France; Partnership for Structural Biology (PSB), Grenoble, France
-
visa fler...
-
- Del Giudice, Rita (författare)
- Malmö University,Malmö universitet,Biofilms Research Center for Biointerfaces,Institutionen för biomedicinsk vetenskap (BMV)
-
- Paracini, Nicolò (författare)
- Malmö University,Malmö universitet,Biofilms Research Center for Biointerfaces,Institutionen för biomedicinsk vetenskap (BMV)
-
- Roosen-Runge, Felix (författare)
- Malmö University,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
-
- Gerelli, Yuri (författare)
- Institut Laue-Langevin, Grenoble, France,Marche Polytechnic University
-
- Prevost, Sylvain (författare)
- Institut Laue-Langevin, Grenoble, France; Department of Life and Environmental Sciences, Marche Polytechnic University, Ancona, Italy
-
- Voss, John C. (författare)
- Department of Biochemistry and Molecular Medicine, University of California, Davis, Davis, CA, United States
-
- Darwish, Tamim A. (författare)
- National Deuteration Facility, Australian Nuclear Science and Technology Organisation, Lucas Heights, NSW, Australia
-
- Yepuri, Nageshwar (författare)
- National Deuteration Facility, Australian Nuclear Science and Technology Organisation, Lucas Heights, NSW, Australia
-
- Pichler, Harald (författare)
- Austrian Centre of Industrial Biotechnology, Graz, Austria; Graz University of Technology, Institute of Molecular Biotechnology, NAWI Graz, BioTechMed Graz, Graz, Austria,Austrian Center of Industrial Biotechnology GmbH
-
- Maric, Selma (författare)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory
-
- Forsyth, V. Trevor (författare)
- Institut Laue-Langevin, Grenoble, France; Partnership for Structural Biology (PSB), Grenoble, France; Faculty of Natural Sciences, Keele University, Staffordshire, United Kingdom
-
- Haertlein, Michael (författare)
- Institut Laue-Langevin, Grenoble, France; Partnership for Structural Biology (PSB), Grenoble, France
-
- Cárdenas, Marité (författare)
- Malmö University,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
-
visa färre...
-
(creator_code:org_t)
- 2021-04-29
- 2021
- Engelska.
-
Ingår i: Frontiers in Chemistry. - : Frontiers Media S.A.. - 2296-2646. ; 9
- Relaterad länk:
-
https://doi.org/10.3...
-
visa fler...
-
https://mau.diva-por... (primary) (Raw object)
-
https://www.frontier...
-
http://dx.doi.org/10... (free)
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
https://lup.lub.lu.s...
-
visa färre...
Abstract
Ämnesord
Stäng
- Apolipoprotein E (ApoE), an important mediator of lipid transportation in plasma and the nervous system, plays a large role in diseases such as atherosclerosis and Alzheimer's. The major allele variants ApoE3 and ApoE4 differ only by one amino acid. However, this difference has major consequences for the physiological behaviour of each variant. In this paper, we follow (i) the initial interaction of lipid-free ApoE variants with model membranes as a function of lipid saturation, (ii) the formation of reconstituted High-Density Lipoprotein-like particles (rHDL) and their structural characterisation, and (iii) the rHDL ability to exchange lipids with model membranes made of saturated lipids in the presence and absence of cholesterol [1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) or 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) with and without 20 mol% cholesterol]. Our neutron reflection results demonstrate that the protein variants interact differently with the model membranes, adopting different protein conformations. Moreover, the ApoE3 structure at the model membrane is sensitive to the level of lipid unsaturation. Small-angle neutron scattering shows that the ApoE containing lipid particles form elliptical disc-like structures, similar in shape but larger than nascent or discoidal HDL based on Apolipoprotein A1 (ApoA1). Neutron reflection shows that ApoE-rHDL do not remove cholesterol but rather exchange saturated lipids, as occurs in the brain. In contrast, ApoA1-containing particles remove and exchange lipids to a greater extent as occurs elsewhere in the body.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- ApoE isoforms
- lipid exchange
- reconstituted HDL
- model membranes
- neutron reflection
- small-angle neutron scattering
- ApoE isoforms
- lipid exchange
- model membranes
- neutron reflection
- reconstituted HDL
- small-angle neutron scattering
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Waldie, Sarah
-
Sebastiani, Fede ...
-
Moulin, Martine
-
Del Giudice, Rit ...
-
Paracini, Nicolò
-
Roosen-Runge, Fe ...
-
visa fler...
-
Gerelli, Yuri
-
Prevost, Sylvain
-
Voss, John C.
-
Darwish, Tamim A ...
-
Yepuri, Nageshwa ...
-
Pichler, Harald
-
Maric, Selma
-
Forsyth, V. Trev ...
-
Haertlein, Micha ...
-
Cárdenas, Marité
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
Frontiers in Che ...
- Av lärosätet
-
Malmö universitet
-
Lunds universitet