Segregation to non-dividing cells in recombinant Escherichia coli fed-batch fermentation processes

• In Escherichia coli fermentation processes, a drastic drop in viable cell count as measured by the number of colony forming units per ml (c.f.u. ml(-1)) is often observed. This phenomenon was investigated in a process for the production of the recombinant fusion protein, promegapoietin (PMP). After induction, the number of c.f.u. ml(-1) dropped to approximately 10% of its maximum though the biomass concentration continued to increase. Flow cytometric analysis of viability and intracellular concentration of PMP showed that almost all cells were alive and contributed to the production. Thus, the drop in the number of c.f.u. ml(-1) probably reflects a loss of cell division capability rather than cell death.

Ämnesord

NATURVETENSKAP  -- Biologi -- Mikrobiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Microbiology (hsv//eng)

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)

Nyckelord

Apoptosis/physiology

Bioreactors/microbiology

Cell Aggregation/physiology

Cell Survival/physiology

Colony Count; Microbial/*methods

Comparative Study

Escherichia coli/*cytology/*physiology

Fermentation/physiology

Flow Cytometry/methods

Interleukin-3

Mitosis/*physiology

Protein Engineering/*methods

Receptors; Interleukin-3/*biosynthesis/genetics

Recombinant Fusion Proteins/biosynthesis

Research Support; Non-U.S. Gov't

Thrombopoietin/*biosynthesis/genetics

Biochemical process engineering

Publikations- och innehållstyp

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