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High fragmentation ...
High fragmentation in platelet concentrates impacts the activation, procoagulant, and aggregatory capacity of platelets
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- Tynngård, Nahreen (författare)
- Linköpings universitet,Avdelningen för klinisk kemi och farmakologi,Medicinska fakulteten,Institutionen för hälsa, medicin och vård,Region Östergötland, Klinisk kemi,Region Östergötland, Forskningsstrategiska enheten
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- Alshamari, Aseel, 1976- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Clinical Immunology and Transfusion medicine, Faculty of Medicine and Health, Örebro University, Sweden; Cardiovascular Research Centre, School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden,Orebro Univ, Sweden; Orebro Univ, Sweden
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- Sandgren, Per (författare)
- Karolinska Institutet,Karolinska Inst, Sweden
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- Kenny, Dermot (författare)
- Clinical Research Centre, Royal College of Surgeons in Ireland, Dublin, Ireland,Royal Coll Surgeons Ireland, Ireland
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- Vasilache, Ana-Maria (författare)
- Linköpings universitet,Avdelningen för neurobiologi,Medicinska fakulteten,Region Östergötland, Klinisk immunologi och transfusionsmedicin
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- Abedi, Mohammad R. (författare)
- Department of Clinical Immunology and Transfusion medicine, Faculty of Medicine and Health, Örebro University, Sweden,Orebro Univ, Sweden
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- Ramström, Sofia, 1973- (författare)
- Linköpings universitet,Örebro universitet,Institutionen för medicinska vetenskaper,Department of Clinical Chemistry and Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden; Cardiovascular Research Centre, School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden,Avdelningen för klinisk kemi och farmakologi,Medicinska fakulteten,Region Östergötland, Klinisk kemi,Orebro Univ, Sweden
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(creator_code:org_t)
- 2023-01-12
- 2023
- Engelska.
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Ingår i: Platelets. - : Taylor & Francis. - 0953-7104 .- 1369-1635. ; 34:1
- Relaterad länk:
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https://doi.org/10.1...
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Platelets are transfused to patients to prevent bleeding. Since both preparation and storage can impact the hemostatic functions of platelets, we studied platelet concentrates (PCs) with different initial composition in regard to platelet fragmentation and its impact on storage-induced changes in activation potential. Ten whole blood derived PCs were assessed over 7 storage days. Using flow cytometry, platelet (CD41+) subpopulations were characterized for activation potential using activation markers (PAC-1, P-selectin, and LAMP-1), phosphatidylserine (Annexin V), and mitochondrial integrity (DiIC1(5)). Aggregation response, coagulation, and soluble activation markers (cytokines and sGPVI) were also measured. Of the CD41+ events, the PCs contained a median of 82% normal-sized platelets, 10% small platelets, and 8% fragments. The small platelets exhibited procoagulant hallmarks (increased P-selectin and Annexin V and reduced DiIC1(5)). Normal-sized platelets responded to activation, whereas activation potential was decreased for small and abolished for fragments. Five PCs contained a high proportion of small platelets and fragments (median of 28% of CD41+ events), which was significantly higher than the other five PCs (median of 9%). A high proportion of small platelets and fragments was associated with procoagulant hallmarks and decreased activation potential, but, although diminished, they still retained some activation potential throughout 7 days storage.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Nyckelord
- Flow cytometry
- microparticles
- platelet concentrate
- platelet storage
- subpopulations
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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