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Topoisomerase II-α ...
Topoisomerase II-α expression in different cell cycle phases in fresh human breast carcinomas
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- Villman, Kenneth (författare)
- Uppsala universitet,Enheten för onkologi
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- Ståhl, Elisabeth (författare)
- 2Department of Pathology, Örebro University Hospital, Örebro, Sweden
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- Liljegren, Göran (författare)
- Örebro universitet,Hälsoakademin,Department of Surgery, Örebro University Hospital, Örebro, Sweden
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- Tidefelt, Ulf (författare)
- Örebro universitet,Hälsoakademin,Department of Medicine, Örebro University Hospital, Örebro, Sweden; Karolinska Institute, Stockholm, Sweden
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- Karlsson, Mats G. (författare)
- Örebro universitet,Hälsoakademin,2Department of Pathology, Örebro University Hospital, Örebro, Sweden
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(creator_code:org_t)
- Baltimore : Lippincott Williams & Wilkins, 2002
- 2002
- Engelska.
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Ingår i: Modern Pathology. - Baltimore : Lippincott Williams & Wilkins. - 0893-3952 .- 1530-0285. ; 15:5, s. 486-491
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Topoisomerase II-alfa (topo IIalfa) is the key target enzyme for the topoisomerase inhibitor class of anti-cancer drugs. In normal cells, topo IIalfa is expressed predominantly in the S/G2/M phase of the cell cycle. In malignant cells, in vitro studies have indicated that the expression of topo IIalfa is both higher and less dependent on proliferation state in the cell. We studied fresh specimens from 50 cases of primary breast cancer. The expression of topo IIalfa in different cell cycle phases was analyzed with two-parameter flow cytometry using the monoclonal antibody SWT3D1 and propidium iodide staining. The expression of topo IIalfa was significantly higher in the S/G2/M phase of the cell cycle than in the G0/G1 phase in both DNA diploid and DNA nondiploid tumors. In 18 of 21 diploid tumors, and in 25 of 29 nondiploid tumors, >50% of the topo IIalfa–positive cells were in the G0/G1 phase. This significant expression of topo IIalfa in the G0/G1 phase of the cell cycle may have clinically important implications for treatment efficacy of topoisomerase II inhibitors.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Nyckelord
- breast cancer
- cell cycle
- DNA flow cytometry
- topoisomerase IIa
- MEDICINE
- MEDICIN
- Medicin
- Medicine
- Biomedicin
- Biomedicine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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