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Sökning: id:"swepub:oai:DiVA.org:oru-113821" > Comparative Metabol...

Comparative Metabolomics and Microbiome Analysis of Ethanol versus OMNImet/gene•GUT Fecal Stabilization

Isokääntä, Heidi (författare)
Research Center for Infections and Immunity, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland
Pinto da Silva, Lucas (författare)
Turku Bioscience Centre, University of Turku, Tykistönkatu 6A, 20520 Turku, Finland
Karu, Naama (författare)
Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden 2333 CC, The Netherlands
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Kallonen, Teemu (författare)
Department of Clinical Microbiology, Laboratory Division, Turku University Hospital, Kiinamyllynkatu 10 D, 20520 Turku, Finland; Clinical Microbiome Bank, Microbe Center, University Hospital and University of Turku, 20520 Turku, Finland
Aatsinki, Anna-Katariina (författare)
Centre for Population Health Research, University of Turku, Kiinamyllynkatu 10A, 20520 Turku, Finland
Hankemeier, Thomas (författare)
Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden 2333 CC, The Netherlands
Schimmel, Leyla (författare)
Department of Psychiatry and Behavioral Sciences, Duke University, Durham, North Carolina 27708-0187, United States
Diaz, Edgar (författare)
Department of Psychiatry and Behavioral Sciences, Duke University, Durham, North Carolina 27708-0187, United States
Hyötyläinen, Tuulia, 1971- (författare)
Örebro universitet,Institutionen för naturvetenskap och teknik
Dorrestein, Pieter C. (författare)
Center for Microbiome Innovation, University of California, San Diego, La Jolla, California 92093-6607, United States; Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman, La Jolla, California 92093-0657, United States
Knight, Rob (författare)
Center for Microbiome Innovation, University of California, San Diego, La Jolla, California 92093-6607, United States
Oresic, Matej, 1967- (författare)
Örebro universitet,Institutionen för medicinska vetenskaper,Turku Bioscience Centre, University of Turku, Tykistönkatu 6A, 20520 Turku, Finland
Kaddurah-Daouk, Rima (författare)
Department of Psychiatry and Behavioral Sciences, Duke University, Durham, North Carolina 27708-0187, United States
Dickens, Alex M. (författare)
Turku Bioscience Centre, University of Turku, Tykistönkatu 6A, 20520 Turku, Finland; Department of Chemistry, University of Turku, Henrikinkatu 2, 20500 Turku, Finland
Lamichhane, Santosh (författare)
Turku Bioscience Centre, University of Turku, Tykistönkatu 6A, 20520 Turku, Finland
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 (creator_code:org_t)
American Chemical Society (ACS), 2024
2024
Engelska.
Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 96:22, s. 8893-9304
Relaterad länk:
https://doi.org/10.1...
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https://urn.kb.se/re...
https://doi.org/10.1...
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • Metabolites from feces provide important insights into the functionality of the gut microbiome. As immediate freezing is not always feasible in gut microbiome studies, there is a need for sampling protocols that provide the stability of the fecal metabolome and microbiome at room temperature (RT). Here, we investigated the stability of various metabolites and the microbiome (16S rRNA) in feces collected in 95% ethanol (EtOH) and commercially available sample collection kits with specific preservatives OMNImet•GUT/OMNIgene•GUT. To simulate field-collection scenarios, the samples were stored at different temperatures at varying durations (24 h + 4 °C, 24 h RT, 36 h RT, 48 h RT, and 7 days RT) and compared to aliquots immediately frozen at -80 °C. We applied several targeted and untargeted metabolomics platforms to measure lipids, polar metabolites, endocannabinoids, short-chain fatty acids (SCFAs), and bile acids (BAs). We found that SCFAs in the nonstabilized samples increased over time, while a stable profile was recorded in sample aliquots stored in 95% EtOH and OMNImet•GUT. When comparing the metabolite levels between aliquots stored at room temperature and at +4 °C, we detected several changes in microbial metabolites, including multiple BAs and SCFAs. Taken together, we found that storing samples at RT and stabilizing them in 95% EtOH yielded metabolomic results comparable to those from flash freezing. We also found that the overall composition of the microbiome did not vary significantly between different storage types. However, notable differences were observed in the α diversity. Altogether, the stability of the metabolome and microbiome in 95% EtOH provided results similar to those of the validated commercial collection kits OMNImet•GUT and OMNIgene•GUT, respectively.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

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