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Azithromycin increa...
Azithromycin increases chloride efflux from cystic fibrosis airway epithelial cells
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- Oliynyk, Igor (författare)
- Uppsala universitet,Örebro universitet,Hälsoakademin,Uppsala University, Sweden,Institutionen för medicinsk cellbiologi
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- Varelogianni, Georgia (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Schalling, Martin (författare)
- Karolinska Institutet
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- Stenkvist Asplund, Monika (författare)
- Uppsala universitet,Institutionen för kirurgiska vetenskaper
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- Roomans, Godfried M. (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Johannesson, Marie (författare)
- Uppsala universitet,Allmänmedicin och klinisk epidemiologi
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(creator_code:org_t)
- 2009-07-02
- 2009
- Engelska.
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Ingår i: Experimental Lung Research. - : Taylor & Francis. - 0190-2148 .- 1521-0499. ; 35:3, s. 210-221
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- It was investigated whether azithromycin (AZM) stimulates chloride (Cl−) efflux from cystic fibrosis (CF) and non-CF airway epithelial cells, possibly secondary to up-regulation of the multidrug resistance protein (MDR). CF and non-CF human airway epithelial cell lines (CFBE and 16HBE) were treated with 0.4, 4, and 40 μ g/mL AZM for 4 days. Cl− efflux was explored in the presence or absence of specific inhibitors of CFTR and alternative Cl− channels. Six CF patients received AZM (500 mg daily) for 6 months. The percentage of predicted forced vital capacity (FVC%), forced expiratory volume (FEV1%), and the number of acute exacerbations were compared before and after treatment. Nasal biopsies were taken before and after treatment, and mRNA expression of MDR and CFTR was determined by in situ hybridization. A significant dose-dependent increase of Cl− efflux from CFBE cells (but not from 16HBE cells) was observed after AZM treatment. A CFTR inhibitor significantly reduced AZM-stimulated Cl− efflux from CFBE cells. A significant improvement in FEV1%, and fewer exacerbations were observed. AZM treatment did not affect mRNA expression of MDR and CFTR. The stimulation of Cl− efflux could be part of the explanation for the clinical improvement seen among the patients.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- azithromycin
- chloride transport
- cystic fibrosis
- lung function
- Morphology
- Medical cell biology
- pathology
- Medicin
- Medicine
- MEDICINE
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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