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Regulation of human...
Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier
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- Karczewski, Jurgen (författare)
- Host Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, Netherlands
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- Troost, Freddy J. (författare)
- TI Food & Nutr, Wageningen, Netherlands; Div Gastroenterol & Hepatol, Dept Internal Med, Nutr & Toxicol Res Inst Maastricht, Maastricht Univ, Maastricht, Netherlands
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- Konings, Irene (författare)
- Host Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, Netherlands
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- Dekker, Jan (författare)
- TI Food & Nutr, Wageningen, Netherlands
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- Kleerebezem, Michiel (författare)
- TI Food & Nutr, Wageningen, Netherlands; NIZO Food Res, Ede, Netherlands; Microbiol Lab, Univ Wageningen, Wageningen, Netherlands
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- Brummer, Robert (författare)
- Örebro universitet,Hälsoakademin,TI Food & Nutr, Wageningen, Netherlands; Div Gastroenterol & Hepatol, Dept Internal Med, Nutr & Toxicol Res Inst Maastricht, Maastricht Univ, Maastricht, Netherlands
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- Wells, Jerry M. (författare)
- Host Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, Netherlands
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(creator_code:org_t)
- American Physiological Society, 2010
- 2010
- Engelska.
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Ingår i: American Journal of Physiology - Gastrointestinal and Liver Physiology. - : American Physiological Society. - 0193-1857 .- 1522-1547. ; 298:6, s. G851-G859
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Lactobacillus plantarum, a commensal bacterium of humans, has been proposed to enhance the intestinal barrier, which is compromised in a number of intestinal disorders. To study the effect of L. plantarum strain WCFS1 on human barrier function, healthy subjects were administered L. plantarum or placebo in the duodenum for 6 h by means of a feeding catheter. The scaffold protein zonula occludens (ZO)-1 and transmembrane protein occludin were found to be significantly increased in the vicinity of the tight-junction (TJ) structures, which form the paracellular seal between cells of the epithelium. In an in vitro model of the human epithelium, L. plantarum induced translocation of ZO-1 to the TJ region; however, the effects on occludin were minor compared with those seen in vivo. L. plantarum was shown to activate Toll-like receptor 2 (TLR2) signaling, and treatment of Caco-2 monolayers with the TLR2 agonist Pam3-Cys-SK4(PCSK) significantly increased fluorescent staining of occludin in the TJ. Pretreatment of Caco-2 monolayers with L. plantarum or PCSK significantly attenuated the effects of phorbol ester-induced dislocation of ZO-1 and occludin and the associated increase in epithelial permeability. Our results identifying commensal bacterial stimulation of TLR2 in the gut epithelium as a regulator of epithelial integrity have important implications for understanding probiotic mechanisms and the control of intestinal homeostasis.
Nyckelord
- epithelium
- intestine
- MEDICINE
- MEDICIN
- Medicine
- Medicin
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