Gene expression analysis of chicken during development subsequent to PFOS and PFOA exposure

• Perfluorinated compounds have been manufactured for over50 years and have a wide range of applications due to theirsurfactant properties. They have been used as fabricprotectors to repel water and dirt, fire-fighting foams, nonstickcoatings, insecticides and all weather clothing. PFCsare widespread in the environment and are found globally inwildlife. Among the most abundant are perfluorooctanesulfonate (PFOS) and perfluorooctanoic acid (PFOA). Thelevels in common guillemot eggs from the Baltic Sea areamong the highest found in the Scandinavian environment.Previous studies show effects on embryo survival of chickenat levels close to levels found in the Baltic guillemot. Toinvestigate the possible mechanisms of action, microarraygene expression analysis was conducted. Chicken eggs wereincubated at 37.5 degrees and 60% relative humidity. PFOSor PFOA was administered to eggs on day 4 of incubationby injection into the air cell of the egg. 1 μl injectionsolution per gram egg was used. The injection solutionscontained PFOS or PFOA dissolved at differentconcentrations in sterile water with 5% or 2.5% dimethylsulfoxide respectively. The doses were 10 and 3 mg/kg forPFOS and 1.6 and 0.5 mg/kg for PFOA. The vehiclesolutions were used as control treatment. At day 19 theembryos were sacrificed and the liver was extracted. Four livers per dose and each vehicle control were used. RNA wasextracted from the livers using Qiagen RNeasy Mini Kit.cDNA was synthesized from mRNA and used for microarrayanalysis on Agilent two-color chicken microarrays.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Biology

Biologi

Biology

Biologi

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)