HIV, Chlamydia trachomatis and Helicobacter pylori vaccine antigen and proteinaceous adjuvant production in arabidopsis and carrot

• In our project, we use plants for synthesis of vaccine antigens and adjuvant proteins. Among targeted diseases are HIV, Chlamydia trachomatis (CT) and Helicobacter pylori (HP) infections. Novel genetic constructs have been designed for production of optimized antigen proteins and several transformation techniques and intracellular protein production sites are being examined for yield optimization. Both Arabidopsis thaliana (HIV, CT, HP) and carrot (HIV, CT) have been successfully used as production hosts and plant-produced antigens against HIV (primarily the p24 protein), CT (own designed chimera of the MOMP protein) and HP (different versions of the TonB protein). For HIV and CT these antigens have been used in immunization trials in laboratory mice, giving rise to systemic immune responses. For this purpose both consumption of plant tissue and distribution of purified antigens have been used. For CT, the administration of the recombinant protein has also been shown to protect against the disease in mice. Moreover, to further increase the effect of vaccine antigens, we also use plants to produce protein-based adjuvants for inclusion in vaccine formulations. These adjuvants are based either on the bacterial flagellin protein or are cholera toxin-derived chimeric proteins.

Ämnesord

LANTBRUKSVETENSKAPER  -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)

AGRICULTURAL SCIENCES  -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)

Nyckelord

Biochemistry

Biokemi

Publikations- och innehållstyp

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