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Vectorial secretion...
Vectorial secretion of interleukin-8 mediates autocrine signalling in intestinal epithelial cells via apically located CXCR1
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- Rossi, Oriana (författare)
- Host-Microbe Interactomics Group, ASG, University of Wageningen, Wageningen, The Netherlands
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- Karczewski, Jurgen (författare)
- Host-Microbe Interactomics Group, ASG, University of Wageningen, Wageningen, The Netherlands
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- Stolte, Ellen H (författare)
- Host-Microbe Interactomics Group, ASG, University of Wageningen, Wageningen, The Netherlands
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- Brummer, Robert J M, 1957- (författare)
- Örebro universitet,Institutionen för läkarutbildning
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- van Nieuwenhoven, Michiel A, 1965- (författare)
- Örebro universitet,Institutionen för hälsovetenskap och medicin
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- Meijerink, Marjolein (författare)
- Host-Microbe Interactomics Group, ASG, University of Wageningen, Wageningen, The Netherlands
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- van Neerven, Joost R J (författare)
- FrieslandCampina, Amersfoort, The Netherlands
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- van Ijzendoorn, Sven C D (författare)
- Department of Cell Biology, Section Membrane Cell Biology, University Medical Center Groningen, Groningen, The Netherlands
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- van Baarlen, Peter (författare)
- Host-Microbe Interactomics Group, ASG, University of Wageningen, Wageningen, The Netherlands
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- Wells, Jerry M (författare)
- Host-Microbe Interactomics Group, ASG, University of Wageningen, Wageningen, The Netherlands
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(creator_code:org_t)
- 2013-10-28
- 2013
- Engelska.
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Ingår i: BMC research notes. - : Springer Science and Business Media LLC. - 1756-0500. ; 6, s. 431-
- Relaterad länk:
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https://bmcresnotes....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- BACKGROUND: In the intestinal mucosa, several adaptations of TLR signalling have evolved to avoid chronic inflammatory responses to the presence of commensal microbes. Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus.RESULTS: In the Caco-2 BBE model of polarized villus-like epithelium, apical stimulation with TLR2 and TLR5 ligands resulted in the apical secretion of IL-8. The CXCR1 receptor for IL-8 was expressed only on the apical membrane of Caco-2 BBE cells and differentiated epithelial cells in the human small intestine and colon. Transcriptome analyses revealed that Caco-2 BBE cells respond to stimulation with IL-8 supporting the hypothesis that IL-8 induces G protein-coupled receptor signalling.CONCLUSIONS: These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo, suggesting an autocrine function for IL-8 secreted in the lumen.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- Intestinal epithelial cells; Interleukin-8; CXCR1; Caco-2; Epithelial repair; Autocrine
- Medicine
- Medicin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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