Sökning: id:"swepub:oai:DiVA.org:oru-34261" >
The Toll-like recep...
The Toll-like receptor 2/1 (TLR2/1) complex initiates human platelet activation via the src/Syk/LAT/PLC gamma 2 signalling cascade
-
- Fälker, Knut, 1971- (författare)
- Linköpings universitet,Örebro universitet,Institutionen för hälsovetenskap och medicin,Department of Biomedicine; Dept Clin & Expt Med, Linköping Univ, Linköping, Sweden,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet
-
- Klarström-Engström, Kristin, 1986- (författare)
- Örebro universitet,Institutionen för hälsovetenskap och medicin,Department of Biomedicine,University of Örebro, Sweden
-
- Bengtsson, Torbjörn, 1955- (författare)
- Örebro universitet,Institutionen för hälsovetenskap och medicin,Department of Biomedicine,University of Örebro, Sweden
-
visa fler...
-
- Lindahl, Tomas (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Hälsouniversitetet,Klinisk kemi
-
- Grenegård, Magnus, 1963- (författare)
- Örebro universitet,Institutionen för läkarutbildning,Department of Biomedicine,University of Örebro, Sweden
-
visa färre...
-
(creator_code:org_t)
- New York, USA : Elsevier, 2014
- 2014
- Engelska.
-
Ingår i: Cellular Signalling. - New York, USA : Elsevier. - 0898-6568 .- 1873-3913. ; 26:2, s. 279-286
- Relaterad länk:
-
http://liu.diva-port...
-
visa fler...
-
https://liu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- The specific TLR2/1 complex activator Pam3CSK4 has been shown to provoke prominent activation and aggregation of human non-nucleated platelets. As Pam3CSK4-evoked platelet activation does not employ the major signalling pathway established in nucleated immune cells, we investigated if the TLR2/1 complex on platelets may initiate signalling pathways known to be induced by physiological agonists such as collagen via GPVI or thrombin via PARs. We found that triggering TLR2/1 complex-signalling with Pam3CSK4, in common with that induced via GPVI, and in contrast to that provoked by PARS, involves tyrosine phosphorylation of the adaptor protein LAT as well as of PLC gamma 2 in a src- and Syk-dependent manner. In this respect, we provide evidence that Pam3CSK4 does not cross-activate GPVI. Further, by the use of platelets from a Glanzmann's thrombasthenia patient lacking beta(3), in contrast to findings in nucleated immune cells, we show that the initiation of platelet activation by Pam3CSK4 does not involve integrin beta(3) signalling; whereas the latter, subsequent to intermediate TXA2 synthesis and signalling, was found to be indispensable for proper dense granule secretion and full platelet aggregation. Together, our findings reveal that triggering the TLR2/1 complex with Pam3CSK4 initiates human platelet activation by engaging tyrosine kinases of the src family and Syk, the adaptor protein LAT, as well as the key mediator PLC gamma 2. (C) 2013 Elsevier Inc. All rights reserved.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- Human platelets
- TLR2
- Pam3CSK4
- Syk
- LAT
- PLC gamma 2
- Cellforskning
- Cell Research
- MEDICINE
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas