Estrogen- and androgen-sensitive bioassays based on primary cell and tissue slice cultures from three-spined stickleback (Gasterosteus aculeatus)

• Endocrine disrupting compounds are chemicals that may interfere with the endocrine system causing severe effects in organisms. The three-spined stickleback (Gasterosteus aculeatus L.) offers a potential for the assessment of endocrine disruption caused by a) estrogenic xenobiotics through the estrogen-dependent protein vitellogenin and b) androgenic xenobiotics through the androgen-dependent protein spiggin. The stickleback is presently the only known fish species with a quantifiable androgen and anti-androgen biomarker endpoint. In the current study, hepatocyte and kidney primary cell cultures and liver and kidney tissue slice cultures were prepared and used for detecting estrogenic or androgenic activity in vitro through the action of hormones or municipal sewage water. The results indicate that stickleback male hepatocyte cultures are suitable in detecting estrogenic activity and stickleback female kidney tissue slice cultures in detecting androgenic activity. The tested sewage water showed high estrogenic activity but no significant androgenic activity. Primary cell and tissue slice cultures isolated from the three-spined stickleback will allow simultaneously screening in vitro for potential estrogenic and androgenic activity of complex samples.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Androgens/*toxicity

Animals

Biological Assay

Biological Markers/metabolism

Cells; Cultured

Estrogens/*toxicity

Female

Fish Proteins/metabolism

Hepatocytes/drug effects/metabolism

Hormone Antagonists/*toxicity

Kidney/drug effects/metabolism

Liver/drug effects/metabolism

Male

Sewage/adverse effects

Smegmamorpha/*physiology

Tissue Culture Techniques

Vitellogenins/metabolism

Water Pollutants; Chemical/*toxicity

NATURAL SCIENCES

NATURVETENSKAP

Chemistry

Kemi

Biochemistry

Biokemi

Biochemistry

Biokemi

Publikations- och innehållstyp

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