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Optimisation and co...
Optimisation and comparison of liquid and dry formulations of the biocontrol yeast Pichia anomala J121
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- Melin, Petter (författare)
- Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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- Hakansson, Sebastian (författare)
- Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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- Schnürer, Johan, 1957- (författare)
- Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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(creator_code:org_t)
- 2006-08-25
- 2007
- Engelska.
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Ingår i: Applied Microbiology and Biotechnology. - New York, USA : Springer. - 0175-7598 .- 1432-0614. ; 73:5, s. 1008-1016
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The biocontrol yeast Pichia anomala J121 can effectively reduce mould growth on moist cereal grains during airtight storage. Practical use of microorganisms requires formulated products that meet a number of criteria. In this study we compared different formulations of P. anomala. The best way to formulate P. anomala was freeze-drying. The initial viability was as high as 80%, with trehalose previously added to the yeast. Freeze-dried products could be stored at temperatures as high as 30 degrees C for a year, with only a minor decrease in viability. Vacuum-drying also resulted in products with high storage potential, but the products were not as easily rehydrated as freeze-dried samples. Upon desiccating the cells using fluidised-bed drying or as liquid formulations, a storage temperature of 10 degrees C was required to maintain viability. Dependent on the type of formulation, harvesting of cells at different nutritional stresses affected the initial viabilities, e.g. the initial viability for fluidised-bed-dried cells was higher when the culture was fed with excess glucose, but for freeze-drying it was superior when cells were harvested after depletion of carbon. Using micro-silos we found that the biocontrol activity remained intact after drying, storage and rehydration for all formulations.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- Pichia anomala
- biocontrol
- formulation
- shelf-life
- trehalose
Publikations- och innehållstyp
- ref (ämneskategori)
- for (ämneskategori)
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