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Mechanistic Links U...
Mechanistic Links Underlying the Impact of C-Reactive Protein on Muscle Mass in Elderly
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- Wåhlin-Larsson, Britta, 1959- (författare)
- Örebro universitet,Institutionen för hälsovetenskaper
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- Wilkinson, Daniel J. (författare)
- MRC-ARUK Centre of Excellence for Musculoskeletal Ageing Research, Division of Medical Sciences and Graduate Entry Medicine, University of Nottingham, Royal Derby Hospital Centre, Derby, United Kingdom
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- Strandberg, Emelie, 1986- (författare)
- Örebro universitet,Institutionen för hälsovetenskaper
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- Hosford-Donovan, Adrian (författare)
- School of Health and Medical Sciences, Örebro University, Örebro, Sweden
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- Atherton, Philip J. (författare)
- MRC-ARUK Centre of Excellence for Musculoskeletal Ageing Research, Division of Medical Sciences and Graduate Entry Medicine, University of Nottingham, Royal Derby Hospital Centre, Derby, United Kingdom
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- Kadi, Fawzi, 1970- (författare)
- Örebro universitet,Institutionen för hälsovetenskaper
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(creator_code:org_t)
- 2017-11-09
- 2017
- Engelska.
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Ingår i: Cellular Physiology and Biochemistry. - : Karger. - 1015-8987 .- 1421-9778. ; 44:1, s. 267-278
- Relaterad länk:
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https://doi.org/10.1...
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https://www.karger.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- BACKGROUND/AIMS: Mechanisms underlying the relationship between systemic inflammation and age-related decline in muscle mass are poorly defined. The purpose of this work was to investigate the relationship between the systemic inflammatory marker CRP and muscle mass in elderly and to identify mechanisms by which CRP mediates its effects on skeletal muscle, in-vitro.METHODS: Muscle mass and serum CRP level were determined in a cohort of 118 older women (67±1.7 years). Human muscle cells were differentiated into myotubes and were exposed to CRP. The size of myotubes was determined after immunofluorescent staining using troponin. Muscle protein synthesis was assessed using stable isotope tracers and key signalling pathways controlling protein synthesis were determined using western-blotting.RESULTS: We observed an inverse relationship between circulating CRP level and muscle mass (β= -0.646 (95% CI: -0.888, -0.405) p<0.05) and demonstrated a reduction (p < 0.05) in the size of human myotubes exposed to CRP for 72 h. We next showed that this morphological change was accompanied by a CRP-mediated reduction (p < 0.05) in muscle protein fractional synthetic rate of human myotubes exposed to CRP for 24 h. We also identified a CRP-mediated increased phosphorylation (p<0.05) of regulators of cellular energy stress including AMPK and downstream targets, raptor and ACC-β, together with decreased phosphorylation of Akt and rpS6, which are important factors controlling protein synthesis.CONCLUSION: This work established for the first time mechanistic links by which chronic elevation of CRP can contribute to age-related decline in muscle function.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Nyckelord
- Ageing; AMPK; Akt/mTOR; Chronic Inflammation; CRP; Myoblast; Protein Synthesis; Skeletal Muscle Cell; Old women
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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