Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry

• Flow cytometry is a method that allows high throughput analysis of individual cells in suspension. By inclusion of fluorescently labelled antibodies, it is possible to analyze the abundance of one or more surface antigens, such as LAMP-1, without prior lysis of cells. Here we describe the special considerations required for the investigation of lysosomal exocytosis from platelets analyzed with flow cytometry.

Ämnesord

NATURVETENSKAP  -- Biologi -- Immunologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Immunology (hsv//eng)

NATURVETENSKAP  -- Biologi -- Cellbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)

Nyckelord

Flow cytometry

Granule

Lysosomal exocytosis

Lysosomal-associated membrane protein-1 (LAMP-1)

Lysosome

Platelet

Platelet activation

Platelet secretion

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)