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Biotinylation Assay...
Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes
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- Samuelsson, Malin (författare)
- Lund University,Lunds universitet,Leukocyt migration,Forskargrupper vid Lunds universitet,Leukocyte Migration,Lund University Research Groups
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- Svensson, Lena, 1973- (författare)
- Örebro University,Lund University,Lunds universitet,Örebro universitet,Institutionen för medicinska vetenskaper,Department of Experimental Medical Science, Lund University, Lund, Sweden;,Leukocyt migration,Forskargrupper vid Lunds universitet,Leukocyte Migration,Lund University Research Groups
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(creator_code:org_t)
- 2019-01-05
- 2019
- Engelska.
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Ingår i: Methods in Molecular Biology. - New York, NY : Humana Press. - 1064-3745 .- 1940-6029. ; 1930, s. 115-122
- Relaterad länk:
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http://dx.doi.org/10...
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visa fler...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- The cycles of internalization of the cell surface β2 integrin receptor lymphocyte function-associated antigen 1 (LFA-1) and its re-exposure on the plasma membrane are important for T-cell trafficking. Biotinylation of cells enables to measure surface expression of receptors, and after reducing surface biotin with reducing buffer, enables to measure the internalization of receptors. Here, by using biotin in combination with reducing buffer and recombinant intercellular adhesion molecule-1 (rICAM-1)-coated dishes and subsequent Western immunoblot analysis, we describe how to measure internalization of the LFA-1 receptor and its re-expression back to the cell surface in motile T-lymphocytes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Biotinylation
- LFA-1 recycling
- T-cell migration
- Biotinylation
- LFA-1 recycling
- T-cell migration
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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