Sökning: id:"swepub:oai:DiVA.org:oru-71653" >
Inflammatory respon...
Inflammatory responses after vitrectomy with vitreous substitutes in a rabbit model
-
- Barth, Henrik (författare)
- Lund University,Lunds universitet,Translationell retinaforskning,Forskargrupper vid Lunds universitet,Biomimetic Retinal Tissue Engineering,Lund University Research Groups
-
- Crafoord, Sven, 1950- (författare)
- Örebro University,Örebro universitet,Institutionen för medicinska vetenskaper,Department of Ophthalmology, Faculty of Medicine and Health, Örebro University Hospital, Orebro, Sweden
-
- Arnér, Karin (författare)
- Lund University,Lunds universitet,Oftalmologi, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Ophthalmology, Lund,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine
-
visa fler...
-
- Ghosh, Fredrik (författare)
- Lund University,Lunds universitet,Translationell retinaforskning,Forskargrupper vid Lunds universitet,Biomimetic Retinal Tissue Engineering,Lund University Research Groups
-
visa färre...
-
(creator_code:org_t)
- 2019-01-17
- 2019
- Engelska.
-
Ingår i: Graefe's Archives for Clinical and Experimental Ophthalmology. - : Springer. - 0721-832X .- 1435-702X. ; 257:4, s. 769-783
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://link.springe...
-
http://dx.doi.org/10... (free)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://lup.lub.lu.s...
-
visa färre...
Abstract
Ämnesord
Stäng
- PURPOSE: To investigate the inflammatory response of current and future potential vitreous substitutes in an experimental in vivo vitrectomy model.METHODS: Twenty-five gauge pars plana vitrectomy was performed in the right eye of 60 pigmented rabbits, with subsequent injection of 0.5-1.0 ml of Healaflow® (cross-linked hyaluronic acid, n = 12), Bio-Alcamid® (polyalkylimide, n = 8), silicone oil (n = 12), or balanced saline solution (BSS, n = 28). Postoperative clinical evaluation was performed; and the rabbits were sacrificed at 1 day, 1 week, or 1 month. The eyecups were then examined macroscopically; the retinas sectioned and stained with hematoxylin and eosin (Htx), and immunohistochemically labeled for glial fibrillary acidic protein (GFAP), CD45, galectin-3, CD68, and CD20. Unoperated left eyes from treated animals as well as eyes from untreated animals were used as controls.RESULTS: Vitrectomy without major complications was achieved in 46/60 eyes. The remaining 14 eyes were analyzed separately. One eye developed endophthalmitis after 1 week and was excluded. Eyes treated with Healaflow®, silicone oil, and BSS had a comparable appearance macroscopically and in Htx-stained sections, whereas Bio-Alcamid®-injected eyes exhibited increased macroscopic inflammation and severely affected retinas. GFAP upregulation was present in all treatment groups, most prominent in eyes treated with Bio-Alcamid® and silicone oil. Upregulation of CD45 and CD68 in the inner retina and vitreous space was most prominent with Bio-Alcamid® treatment, and these eyes together with their silicone oil-treated counterparts also displayed a stronger upregulation of CD20-labeled cells compared with remaining groups. General upregulation of galectin-3, mainly in the inner retina, was found in all groups. In eyes with perioperative complications, labeling of CD45, CD68, and especially GFAP was comparably high.CONCLUSIONS: We here describe differences in the postsurgery inflammatory profiles of existing and potential vitreous substitutes. Bio-Alcamid® and silicone oil display severe signs of gliosis and inflammation, whereas Healaflow® elicits minimal reactions comparable with BSS, highlighting its potential application as a vitreous substitute in a future clinical setting.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Oftalmologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Ophthalmology (hsv//eng)
Nyckelord
- Hyaluronic acid
- Hydrogel
- Methods
- Vitreoretinal surgery
- Vitreous
- Vitreous substitute
- Hyaluronic acid
- Hydrogel
- Methods
- Vitreoretinal surgery
- Vitreous
- Vitreous substitute
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas