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Platelet function t...
Platelet function testing at low platelet counts : When can you trust your analysis?
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- Boknäs, Niklas, 1979- (författare)
- Linköpings universitet,Avdelningen för klinisk kemi,Medicinska fakulteten,Region Östergötland, Hematologiska kliniken US,Australian Centre for Blood Diseases, Monash University, Melbourne, Australia
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- Macwan, Ankit (författare)
- Linköpings universitet,Avdelningen för klinisk kemi,Medicinska fakulteten
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- Södergren, Anna L., 1985- (författare)
- Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten
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- Ramström, Sofia, 1973- (författare)
- Linköpings universitet,Örebro universitet,Institutionen för medicinska vetenskaper,Department of Clinical Chemistry and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden; Cardiovascular Research Centre, Örebro University, School of Medical Sciences, Örebro, Sweden,Avdelningen för klinisk kemi,Medicinska fakulteten,Region Östergötland, Klinisk kemi
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(creator_code:org_t)
- Wiley-Blackwell, 2019
- 2019
- Engelska.
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Ingår i: Research and Practice in Thrombosis and Haemostasis. - : Wiley-Blackwell. - 2475-0379. ; 3:2, s. 285-290
- Relaterad länk:
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https://doi.org/10.1...
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https://onlinelibrar...
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: Although flow cytometry is often brought forward as a preferable method in the setting of thrombocytopenia, the relative effects of low sample counts on results from flow cytometry-based platelet function testing (FC-PFT) in comparison with light transmission aggregometry (LTA) and multiple electrode aggregometry (MEA) has not been reported.Objectives: To compare the effects of different sample platelet counts (10, 50, 100, and 200x10(9)L(-1)) on platelet activation measured with FC-PFT, LTA, and MEA using the same anticoagulant and agonist concentrations as for the commercial MEA test.Methods: Platelets were stimulated with two commonly used platelet agonists (ADP [6.5 mu molL(-1)] and PAR1-AP [TRAP, 32 mu molL(-1)]). The specified sample platelet counts were obtained by combining platelet-rich and platelet poor hirudinized plasma in different proportions with or without red blood cells.Results: For FC, P-selectin exposure and PAC-1 binding was reduced at 10x10(9)L(-1) after stimulation with PAR1-AP (by approximately 20% and 50%, respectively), but remained relatively unchanged when ADP was used as agonist (n=9). The platelet count-dependent effects observed with PAR1-AP were eliminated when samples were pre-incubated with apyrase, implying that reduced purinergic signaling was the main underlying factor (n=5). Both aggregometry-based PFTs showed a 50% reduction at 50x10(9)L(-1) and more than 80% reduction at 10x10(9)L(-1), irrespective of agonist used (n=7).Conclusions: Although FC-PFT is generally preferable to aggregometry-based PFTs in situations with low sample platelet counts, a careful optimization of experimental parameters is still required in order to eliminate platelet count-related effects.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- platelet activation
- platelet aggregation
- platelet count
- platelet function tests
- thrombocytopenia
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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