Peritoneal dialysis fluid-induced angiogenesis in rat mesentery is increased by lactate in the presence or absence of glucose

• Angiogenesis may be an important mechanism behind the functional deterioration of the peritoneum leading to ultrafiltration failure in peritoneal dialysis. The present study was designed to compare the angiogenic properties of lactate-, bicarbonate-, and pyruvate-buffered fluids, evaluated separately with and without glucose. Five different fluids (lactate and bicarbonate with and without 2.5% glucose and pyruvate without glucose) were studied for 5 weeks of twice-daily injections in rats. The respective buffers (40 mmol/l) were adjusted to pH 7.2, and sodium, chloride, calcium, and magnesium were present at standard concentrations. The mesenteric window model, based on observation of the translucent peritoneal sections of the small intestine mesentery, was used for immunohistochemical imaging of microvessels (RECA-1 antigen) and macrophages (ED1 and ED2 antigens). All fluids induced angiogenesis as compared with untreated controls. The lactate-buffered fluids induced larger vascularized zones than did their bicarbonate- and pyruvate-buffered counterparts. Angiogenesis was accompanied by a local recruitment of ED1 macrophages from blood. Addition of glucose to the lactate- and bicarbonate-buffered fluids did not seem to alter their pro-angiogenic properties. In conclusion, intraperitoneal exposure to lactate buffer, compared with bicarbonate, stimulates angiogenesis in the presence or absence of glucose.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)

Nyckelord

Antigens

Fluid mechanics

Genetic engineering

Glucose

Immunology

Medical imaging

pH effects

Fluid-induced angiogenesis

Peritoneal dialysis

Ultrafiltration failure

Blood vessel prostheses

antigen

bicarbonate

buffer

calcium

cell antigen

ed 1 antigen

ed 2 antigen

lactic acid

magnesium

peritoneal dialysis fluid

pyruvic acid

reca 1 antigen

sodium chloride

unclassified drug

dialysis fluid

lactic acid derivative

angiogenesis

animal experiment

animal model

animal tissue

article

concentration (parameters)

controlled study

diagnostic imaging

imaging system

immunohistochemistry

macrophage

male

mesentery

microvasculature

nonhuman

pH

rat

small intestine

animal

comparative study

drug effect

fluorescence microscopy

methodology

peritoneum macrophage

Sprague Dawley rat

time

vascularization

Animals

Bicarbonates

Buffers

Dialysis Solutions

Hydrogen-Ion Concentration

Lactates

Macrophages

Peritoneal

Microscopy

Fluorescence

Neovascularization

Physiologic

Rats

Rats

Sprague-Dawley

Time Factors

Animals

Bicarbonates

pharmacology

Buffers

Dialysis Solutions

Glucose

Hydrogen-Ion Concentration

Immunohistochemistry

Lactates

Macrophages

Peritoneal

drug effects

Male

Mesentery

blood supply

Microscopy

Fluorescence

Neovascularization

Physiologic

Peritoneal Dialysis

methods

Pyruvic Acid

Rats

Sprague-Dawley

Time Factors

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)