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Molecular investiga...
Molecular investigations on a chimeric strain of Staphylococcus aureus sequence type 80
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- Gawlik, Darius (author)
- Institute of Infectious Diseases and Infection Control, University Hospital, Jena, Germany; PTC-Phage Technology Center GmbH, Bönen, Germany
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- Ruppelt-Lorz, Antje (author)
- Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany
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- Müller, Elke (author)
- Leibniz Institute of Photonic Technology (IPHT), Jena, Germany; InfectoGnostics Research Campus, Jena, Germany
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- Reißig, Annett (author)
- Leibniz Institute of Photonic Technology (IPHT), Jena, Germany; InfectoGnostics Research Campus, Jena, Germany
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- Hotzel, Helmut (author)
- Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Jena, Germany
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- Braun, Sascha D. (author)
- Leibniz Institute of Photonic Technology (IPHT), Jena, Germany; InfectoGnostics Research Campus, Jena, Germany
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- Söderquist, Bo, 1955- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Laboratory Medicine, Clinical Microbiology
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- Ziegler-Cordts, Albrecht (author)
- T-Systems Multimedia Solutions GmbH, Dresden, Germany
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- Stein, Claudia (author)
- Institute of Infectious Diseases and Infection Control, University Hospital, Jena, Germany
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- Pletz, Mathias W. (author)
- Institute of Infectious Diseases and Infection Control, University Hospital, Jena, Germany
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- Ehricht, Ralf (author)
- Leibniz Institute of Photonic Technology (IPHT), Jena, Germany; InfectoGnostics Research Campus, Jena, Germany; Institute of Physical Chemistry, Jena University, Jena, Germany
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- Monecke, Stefan (author)
- Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany; Leibniz Institute of Photonic Technology (IPHT), Jena, Germany; InfectoGnostics Research Campus, Jena, Germany
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(creator_code:org_t)
- 2020-10-14
- 2020
- English.
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In: PLOS ONE. - : PLOS. - 1932-6203. ; 15:10
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Abstract
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- A PVL-positive, methicillin-susceptible Staphylococcus aureus was cultured from pus from cervical lymphadenitis of a patient of East-African origin. Microarray hybridisation assigned the isolate to clonal complex (CC) 80 but revealed unusual features, including the presence of the ORF-CM14 enterotoxin homologue and of an ACME-III element as well as the absence of etD and edinB. The isolate was subjected to both, Illumina and Nanopore sequencing allowing characterisation of deviating regions within the strain´s genome. Atypical features of this strain were attributable to the presence of two genomic regions that originated from other S. aureus lineages and that comprised, respectively, 3% and 1.4% of the genome. One deviating region extended from walJ to sirB. It comprised ORF-CM14 and the ACME-III element. A homologous but larger fragment was also found in an atypical S. aureus CC1/ST567 strain whose lineage might have served as donor of this genomic region. This region itself is a chimera comprising fragments from CC1 as well as fragments of unknown origin. The other deviating region comprised the region from htsB to ecfA2, i.e., another 3% of the genome. It was very similar to CC1 sequences. Either this suggests an incorporation of CC1 DNA into the study strain, or alternatively a recombination event affecting "canonical" CC80. Thus, the study strain bears witness of several recombination events affecting supposedly core genomic genes. Although the exact mechanism is not yet clear, such chimerism seems to be an additional pathway in the evolution of S. aureus. This could facilitate also a transmission of virulence and resistance factors and therefore offer an additional evolutionary advantage.
Subject headings
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Gawlik, Darius
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Ruppelt-Lorz, An ...
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Müller, Elke
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Reißig, Annett
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Hotzel, Helmut
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Braun, Sascha D.
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show more...
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Söderquist, Bo, ...
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Ziegler-Cordts, ...
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Stein, Claudia
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Pletz, Mathias W ...
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Ehricht, Ralf
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Monecke, Stefan
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
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PLOS ONE
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Örebro University