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Diastereomers of th...
Diastereomers of the Brominated Flame Retardant 1,2-dibromo-4-(1,2 dibromoethyl)cyclohexane Induce Androgen Receptor Activation in the HepG2 Hepatocellular Carcinoma Cell Line and the LNCaP Prostate Cancer Cell Line
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- Khalaf, Hazem (author)
- Örebro universitet,Akademin för naturvetenskap och teknik
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- Larsson, Anders (author)
- Örebro universitet,Akademin för naturvetenskap och teknik
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- Berg, Håkan (author)
- Örebro universitet,Akademin för naturvetenskap och teknik
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- McCrindle, Robert (author)
- Wellington Laboratories Inc., Research Division, Guelph, Ontario, Canada
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- Arsenault, Gilles (author)
- Wellington Laboratories Inc., Research Division, Guelph, Ontario, Canada
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- Olsson, Per-Erik (author)
- Örebro universitet,Akademin för naturvetenskap och teknik
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(creator_code:org_t)
- National Institute of Environmental Health Science, 2009
- 2009
- English.
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In: Journal of Environmental Health Perspectives. - : National Institute of Environmental Health Science. - 0091-6765 .- 1552-9924. ; 117:12, s. 1853-1859
- Related links:
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Background: Reported incidences of prostate cancer and masculinization of animals indicate a release of compounds with androgenic properties into the environment. Large numbers of environmental pollutants have been screened to identify such compounds; however, not until recently was 1,2-dibromo-4-(1,2-dibromoethyl)cyclohexane (TBECH) identified as the first potent activator of the human androgen receptor (hAR). TBECH has been found in beluga whales and bird eggs and has also been found to be maternally transferred in zebrafish.Objectives: In the present study we investigated interaction energies between TBECH diastereomers (α, β, γ, and δ) and the hAR, and their ability to activate the receptor and induce prostate-specific antigen (PSA) expression in vitro.Methods: We performed computational modeling to determine interaction energies between the ligand and the AR ligand-binding site, and measured in vitro competitive binding assays for AR by polarization fluorometry analysis. We used enzyme-linked immunosorbent assays to determine PSA activity in LNCaP and HepG2 cells.Results: We found the γ and δ diastereomers to be more potent activators of hAR than the α and β diastereomers, which was confirmed in receptor binding studies. All TBECH diastereomers induced PSA expression in LNCaP cells even though the AR present in these cells is mutated (T877A). Modeling studies of LNCaP AR revealed that TBECH diastereomers bound to the receptor with a closer distance to the key amino acids in the ligand-binding domain, indicating stronger binding to the mutated receptor.Conclusions: The present study demonstrates the ability of TBECH to activate the hAR, indicating that it is a potential endocrine disruptor.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Miljövetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Environmental Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- androgen
- brominated flame retardant
- endocrine disruptor
- NATURAL SCIENCES
- NATURVETENSKAP
- Chemistry
- Kemi
- Environmental chemistry
- Miljökemi
- Biology
- Biologi
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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